A study of the process of apoptosis in animals infected with the contagious ecthyma virus

Veterinary Microbiology
G I Garrido-FariñaJ Tórtora-Pérez

Abstract

Contagious ecthyma virus (CEV) is a disease caused by a parapoxvirus, also is a potent genetic carrier with the capacity for regulating apoptosis in the cells of infected skin, a mechanism that serves for evading the immune response of the host. It has been suggested that the virus may remain in the skin and be able to cause repeated infections in the same flock. The effect of infection as well as the presence of contagious ecthyma virus was evaluated in terms of lesions and apoptosis in the skin of animals, infected both naturally and experimentally. Samples used were obtained from a naturally infected sheep, 5 goats inoculated with CEV and a negative control. Samples obtained were longitudinally sectioned and processed using photon and electron microscopy, and embedded in paraffin and araldite. Samples embedded in paraffin were sectioned in 5 microm of thickness and dyed with orange eosin-hematoxilin G and Gomori's trichrom stain, apoptosis was demonstrated by the TUNEL assay, the viral antigen was revealed using polyclonal antibodies, and the presence of lymphocytes CD4+ and CD8+, with monoclonal antibodies. The samples processed in resin were cut to obtain semi-fine sections and dyed with toluidine blue-borax, and the ultra...Continue Reading

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Citations

May 30, 2015·Explore : the Journal of Science and Healing·Frederic A Alling
Nov 30, 2011·Journal of Veterinary Science·Shun ChenXiaoyu Wang
Oct 30, 2018·Reviews in Medical Virology·Ruixue WangShuhong Luo
Feb 27, 2015·Journal of Medical Ethics·Lisa Hara Levin, William A Reppy
Sep 9, 2009·Expert Review of Anti-infective Therapy·Madhusudan HosamaniRaj Kumar Singh

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