A study of the regional distribution of bone formed around hydroxyapatite implants in the tibiae of streptozotocin-induced diabetic rats using multiple fluorescent labeling and confocal laser scanning microscopy

Journal of Periodontology
S IyamaT Tanaka

Abstract

The present study was designed to compare the amount and regional distribution of bone formation around hydroxyapatite (HA) implants in normal (control) rats with that of animals with diabetes mellitus (DM), induced by streptozotocin 2 weeks prior to implant placement. Calcein (CAL), alizarin complexone (AL), and tetracycline (TC) were injected on the 7th, 14th, and 21st days after implantation, respectively, and the rats were sacrificed on the 28th day after implantation. Seventy-microns undecalcified sections of the HA-bone interface in both groups were then prepared for confocal laser scanning microscopy (CLSM) observation. In both groups, bone formation developed from the HA surface to the endosteum, periosteum, or bone marrow. In the control group, around the HA close to the endosteum and periosteum, the new bone showed an extensive lamination pattern of three color layers (CAL, AL, and TC), but in the DM group the labeling density of TC on the 21st day was low. In contrast, on the lateral part of the HA surface (away from the endosteum and periosteum), there was considerably less bone formation in the control group, and in the DM group it was almost completely suppressed. These findings indicate that bone formation around...Continue Reading

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Citations

Oct 17, 2003·Implant Dentistry·José T SiqueiraPaulina Sannomiya
Sep 29, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Toru IshibeTetsu Takahashi
Sep 9, 2006·Clinical Oral Implants Research·Sotirios KotsovilisIoannis Fourmousis
May 4, 2012·Microscopy Research and Technique·Ariadna García-HerraizJosé García-Antón
Nov 19, 2011·Clinical Oral Implants Research·Daisuke EsakiKiyoshi Koyano
May 15, 2010·Clinical Oral Implants Research·M Retzepi, N Donos
Aug 27, 2010·Journal of Biomedical Materials Research. Part a·Lei ZhangKunio Ishikawa
Jul 26, 2012·Journal of Clinical Periodontology·Peterson Antônio Dutra OliveiraPatricia Ramos Cury
Nov 25, 2011·Clinical Oral Implants Research·K A SchlegelC von Wilmowsky
Nov 20, 2009·Clinical Oral Implants Research·Maria RetzepiNikolaos Donos
Mar 4, 2014·Dental Materials Journal·Takuma SuzukiKazuhiro Gomi
Apr 29, 2005·Journal of Periodontology·Peter T KwonJoseph P Fiorellini
Feb 7, 2008·Journal of Periodontology·Alethéia B PablosPatricia R Cury
Aug 9, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Thomas Beikler, Thomas F Flemmig
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Aug 25, 2020·Injury·Marianne Comeau-GauthierGeraldine Merle
Mar 25, 2018·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Paulo G CoelhoMichel Dard

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