PMID: 3753456Jun 1, 1986Paper

A study of the role of metallothionein in the inherited copper toxicosis of dogs

The Biochemical Journal
D M HuntD M Danks

Abstract

The role of metallothionein (MT) was assessed in the copper-loading disease prevalent in Bedlington terriers. Fractionation of tissue supernatants over Sephadex G-75 showed that most of the additional cytosolic copper present in liver tissue of these dogs was bound to MT, and that substantially more MT-bound copper could be solubilized by detergent plus mercaptoethanol. Zinc contents were only slightly raised, although most of the extra zinc was associated with a 4000-Mr ligand. Ion-exchange chromatography revealed two isoproteins, MT1 and MT2, in all the dog liver samples examined. In Bedlington terrier liver, copper associated with both isoproteins was increased, although the increase for MT2 was greater than for MT1. The content of MT protein was also raised, although cell-free translations and RNA blots of total liver RNA showed that this increase was not associated with a rise in MT mRNA. The significance of these results to the mechanism of copper accumulation in the Bedlington terrier disorder is discussed.

Citations

Mar 19, 2004·Journal of Veterinary Science·Changbaig Hyun, Lucio John Filippich
Mar 1, 1990·Journal of Hepatology·C D BingleO Epstein
Mar 1, 1995·The Veterinary Clinics of North America. Small Animal Practice·D S Rolfe, D C Twedt
Mar 24, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·L P Thornburg
Sep 24, 2005·Basic & Clinical Pharmacology & Toxicology·Ana Rosa LindeKarl Heinz Summer
Jan 1, 1988·Critical Reviews in Food Science and Nutrition·R NathM Sharma
May 1, 1989·Perception & Psychophysics·L J Trainor, S E Trehub
Sep 19, 2019·Metallomics : Integrated Biometal Science·Yoshinari SuzukiIzumi Watanabe
Nov 24, 2020·Metallomics : Integrated Biometal Science·Adyn MelenbacherMartin J Stillman
Feb 5, 2021·Journal of the American Veterinary Medical Association·Sharon A CenterCynthia R L Webster
May 1, 2021·Animals : an Open Access Journal From MDPI·Ana Margarida PereiraAna Rita Jordão Cabrita
Jan 1, 1989·Chemico-biological Interactions·K AgarwalG Talukder

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