A study on OPG/RANK/RANKL axis in osteoporotic bile duct-ligated rats and the involvement of nitrergic and opioidergic systems

Research in Pharmaceutical Sciences
Amir Hossein DoustimotlaghAbolfazl Golestani

Abstract

Chronic liver disease (CLD) affects millions of people and its impact on bone loss has become a subject of interest. Nitric oxide and endogenous opioids are suggested to increase during cholestasis/cirrhosis and may impact bone resorption by different mechanisms. The receptor activator of nuclear factor-κB (RANK)/RANK-ligand (RANKL)/osteoprotegerin (OPG) signaling pathway regulates bone resorption, but its role in metabolic bone disease subsequent to CLD is unknown. We aimed to investigate the involvement of nitrergic and opioidergic systems in bone loss relative to the RANK/RANKL/OPG pathway, in bile duct-ligated (BDL) rats. Eighty BDL/sham-operated (SO) rats received injections of 3 mg/kg/day Nω-Nitro-L-arginine methyl ester ± naltrexone (10 mg/kg/day) or saline for 28 days. Plasma bone turnover markers, OPG, RANK, and RANKL along with mRNA expression levels of the latter three were assessed. Plasma bone turnover markers and OPG level increased, but RANKL decreased in the BDL group compared with their SO controls (both: P ≤ 0.05). Administration of naltrexone reduced bone turnover markers and OPG level while increased RANKL content in comparison to BDL rats (P ≤ 0.05). As compared to untreated BDL rats, nitric oxide inhibitio...Continue Reading

References

Sep 27, 2007·The Journal of Clinical Endocrinology and Metabolism·Damaris VegaKhashayar Sakhaee

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Citations

May 14, 2020·Ear, Nose, & Throat Journal·Agnieszka WiatrMaciej Wiatr
Jun 14, 2020·Molecular Biology Reports·Amir Hossein DoustimotlaghArash Asfaram
Oct 4, 2020·Biotechnology and Applied Biochemistry·Mohsen NiksereshtAmir Hossein Doustimotlagh
Apr 4, 2021·International Journal of Molecular Sciences·Ramsha ShamsNarendra L Banik
May 15, 2021·International Immunopharmacology·Na QuFengping Shan
Jun 12, 2021·Clinical Oral Implants Research·Reza TalebianReinhard Gruber
Jun 18, 2021·Journal of Toxicology·Hossein SadeghiAmir Hossein Doustimotlagh
Aug 10, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhengping ZhangHonghui Sun
Jul 7, 2021·Journal of Biochemical and Molecular Toxicology·Tiansong YangShengyong Bao

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
electrophoresis
PCR

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