A study on the fundamental factors determining the efficacy of siRNAs with high C/G contents

Cellular & Molecular Biology Letters
Jie-Ying LiaoJia-Chang Yue

Abstract

Although there are many reports about the efficacy of siRNAs, it is not clear whether those siRNAs with high C/G contents can be used to silence their target mRNAs efficiently. In this study, we investigated the structure and function of a group of siRNAs with high C/G contents. The results showed that single siRNAs against the Calpain, Otoferlin and Her2 mRNAs could induce different silencing effects on their targets, suggesting that the accessibility to target sequences influences the efficacy of siRNA. Unexpectedly, a single siRNA could target its cognate sequence in the 3'UTR of EEF1D or the 5'UTR of hTRF2 or CDC6. Their interaction induced different modes of gene silencing. Furthermore, the introduction of mutations into the 3' end of the passenger strand showed that the position and number of mutated nucleotides could exert some influence on the efficacy of siRNA. However, these mutations did not completely block the passenger strand from exerting its RNAi effect. Interestingly, our findings also indicated that the target mRNA might play essential roles in maintaining or discarding the guide strand in RISCs. Thus, the conclusion could be drawn that favorable siRNA sequences, accessible target structures and the fast cleav...Continue Reading

References

Jan 30, 1999·Genes & Development·P A Sharp
Feb 7, 2001·Genes & Development·S M ElbashirT Tuschl
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Sayda M ElbashirThomas Tuschl
Dec 26, 2002·The Journal of Biological Chemistry·Timothy A VickersBrenda F Baker
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Oct 22, 2003·Cell·Dianne S SchwarzPhillip D Zamore
Oct 22, 2003·Cell·Anastasia KhvorovaSumedha D Jayasena
Jan 27, 2004·FEBS Letters·Hirohiko Hohjoh
Feb 6, 2004·Nature Biotechnology·Angela ReynoldsAnastasia Khvorova
Feb 6, 2004·Nucleic Acids Research·Koichi YoshinariKazunari Taira
Feb 26, 2004·Nature Structural & Molecular Biology·Michelle A Carmell, Gregory J Hannon
Mar 27, 2004·Biochemical and Biophysical Research Communications·Mohammed Amarzguioui, Hans Prydz
Apr 28, 2004·Biochemical and Biophysical Research Communications·Kathy Q Luo, Donald C Chang
May 1, 2004·Cell Cycle·Shigeru TakasakiAkihiko Konagaya
May 25, 2004·Nature Structural & Molecular Biology·Andreas LingelMichael Sattler
Jun 1, 2004·Nature Structural & Molecular Biology·Benjamin Haley, Phillip D Zamore
Jun 25, 2004·Nucleic Acids Research·Ye DingCharles E Lawrence
Jul 3, 2004·Genes & Development·Katsutomo OkamuraMikiko C Siomi
Jul 31, 2004·Science·Jidong LiuGregory J Hannon
Aug 7, 2004·Science·Kevin V MorrisDavid J Looney
Sep 18, 2004·Cancer Gene Therapy·Tie-gang LiuRong-guang Shao
Oct 12, 2004·Trends in Genetics : TIG·Aimee L Jackson, Peter S Linsley
Nov 20, 2004·Science·Yukihide TomariPhillip D Zamore
Feb 3, 2005·Nucleic Acids Research·Ellen M WesterhoutBen Berkhout
Feb 5, 2005·Trends in Biochemical Sciences·Guiliang Tang
Feb 22, 2005·Nucleic Acids Research·Bret S E HealeJohn J Rossi
Apr 22, 2005·Journal of Molecular Biology·Marita OverhoffIan Robbins
Apr 27, 2005·Nature Structural & Molecular Biology·Kirk M BrownTariq M Rana
Jun 1, 2005·RNA·Bernd JaglaJames E Rothman
Jun 28, 2005·Nucleic Acids Research·P Clote
Jul 29, 2005·Nucleic Acids Research·Scott D RoseMark A Behlke
Mar 3, 2006·Genes & Development·Marco Antonio Valencia-SanchezRoy Parker
Jul 13, 2006·Annual Review of Biomedical Engineering·Derek M Dykxhoorn, Judy Lieberman
Aug 25, 2006·Nature Methods·Yi Pei, Thomas Tuschl
Jul 31, 2007·Cell·Yukihide TomariPhillip D Zamore

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