PMID: 3753444May 1, 1986Paper

A subclass of glutathione S-transferases as intracellular high-capacity and high-affinity steroid-binding proteins

The Biochemical Journal
H HommaI Listowsky

Abstract

The distribution of glucocorticoids incubated with rat liver cytosol preparations or administered in vivo to adrenalectomized rats was analysed by chromatographic procedures. Corticosterone or dexamethasone was co-eluted with Yb-type GSH S-transferases in anion-exchange and gel-permeation chromatography systems, and these glucocorticoids also were bound to Yb forms in analyses by immunoadsorbent and lysyl-GSH affinity matrices. Pretreatment of cytosol with lysyl-GSH to extract GSH S-transferases or incubation with excess bilirubin, which is expected to compete with steroids for binding to the protein, yielded preparations that were devoid of this major steroid-binding component. In mixtures of the multiple rat GSH S-transferases, corticosterone preferentially interacted with Yb forms rather than Ya and Yc subgroups. All of the multiple Yb forms resolved by chromatofocusing procedures retained the steroid-binding capacity. It is suggested that these abundant proteins can account for a considerable share of intracellular glucocorticoid binding and represent a high-affinity non-saturable binding component with potential to function in steroid-hormone metabolism and action.

Citations

Feb 3, 1999·FEBS Letters·M DemeuleR Béliveau
Mar 8, 2003·Archives of Andrology·E E MontielE A Castellon
Nov 1, 1988·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M Abramovitz, I Listowsky
Jan 1, 1994·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A R BartholomaeusJ T Ahokas
Sep 6, 2007·Drug Metabolism Reviews·K Cameron Falkner, Russell A Prough
Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·S Tsuchida, K Sato
Sep 24, 2015·Endocrinology·Mickaël Di-LuoffoJacques J Tremblay
Jan 1, 1990·Chemico-biological Interactions·R M Vos, P J Van Bladeren
Feb 1, 1994·Chemico-biological Interactions·G A LeBlanc
May 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·K S Lin, N N Chuang
Jul 1, 1988·Archives of Biochemistry and Biophysics·S V SinghY C Awasthi
Sep 1, 1989·Archives of Biochemistry and Biophysics·S IshigakiI Listowsky
Jul 22, 2006·Biochemical and Biophysical Research Communications·Alison J ShieldPhilip G Board
Feb 9, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Damodar GullipalliAparna Dutta-Gupta

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.