A subpopulation of rat muscle fibers maintains an assessable excitation-contraction coupling mechanism after long-standing denervation despite lost contractility

Journal of Neuropathology and Experimental Neurology
Roberta SqueccoSandra Zampieri

Abstract

To define the time course and potential effects of electrical stimulation on permanently denervated muscle, we evaluated excitation-contraction coupling (ECC) of rat leg muscles during progression to long-term denervation by ultrastructural analysis, specific binding to dihydropyridine receptors, ryanodine receptor 1 (RYR-1), Ca channels and extrusion Ca pumps, gene transcription and translation of Ca-handling proteins, and in vitro mechanical properties and electrophysiological analyses of sarcolemmal passive properties and L-type Ca current (ICa) parameters. We found that in response to long-term denervation: 1) isolated muscle that is unable to twitch in vitro by electrical stimulation has very small myofibers but may show a slow caffeine contracture; 2) only roughly half of the muscle fibers with "voltage-dependent Ca channel activity" are able to contract; 3) the ECC mechanisms are still present and, in part, functional; 4)ECC-related gene expression is upregulated; and 5) at any time point, there are muscle fibers that are more resistant than others to denervation atrophy and disorganization of the ECC apparatus. These results support the hypothesis that prolonged "resting" [Ca] may drive progression of muscle atrophy to ...Continue Reading

References

May 1, 1987·Journal of Neuropathology and Experimental Neurology·I MussiniU Carraro
Mar 1, 1982·The Journal of Physiology·C A CollinsB A Suarez-Isla
May 8, 1995·Biochimica Et Biophysica Acta·W MelzerH C Lüttgau
Dec 1, 1996·Journal of the Neurological Sciences·L Billington, B M Carlson
Jun 1, 1997·The Journal of Membrane Biology·M RenganathanO Delbono
Aug 1, 1997·Pflügers Archiv : European journal of physiology·M MidrioR Betto
Feb 27, 1999·Journal of Muscle Research and Cell Motility·S BeliaG Fanò
Nov 17, 2001·The Journal of Physiology·F FranciniR Squecco
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 10, 2002·Artificial Organs·Christian HoferHelmut Kern
Dec 9, 2003·The Journal of Physiology·Roberta SqueccoFabio Francini
Jan 16, 2004·Experimental Gerontology·Stefania FulleGiorgio Fanò
Feb 12, 2004·Journal of Muscle Research and Cell Motility·Chiara BenciniFabio Francini
Aug 7, 2004·The Journal of Physiology·Anthony Michael PayneOsvaldo Delbono
Feb 24, 2005·Artificial Organs·Michaela MödlinHelmut Kern
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Oct 1, 2005·Journal of Rehabilitation Research and Development·Helmut KernChristian Hofer
Aug 10, 2006·European Journal of Applied Physiology·Menotti Midrio
Oct 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Marco SandriBruce M Spiegelman
Jan 16, 2007·American Journal of Physiology. Cell Physiology·Z AshleyJ C Jarvis
Jun 23, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Florian L MullerHolly Van Remmen
Jul 14, 2007·The Journal of Physiology·Cecilia PaoliniFeliciano Protasi

❮ Previous
Next ❯

Citations

Jan 26, 2010·Current Opinion in Clinical Nutrition and Metabolic Care·Antonio MusaròGiorgio Fanò
Dec 30, 2014·Frontiers in Cellular Neuroscience·Bruno A CisternaJuan C Sáez
Oct 21, 2015·Oncotarget·Laura PietrangeloFeliciano Protasi
Jan 5, 2011·Journal of Cellular and Molecular Medicine·Chiara SassoliElisabetta Meacci
Jun 14, 2019·Frontiers in Neurology·Pierpaolo IodiceFeliciano Protasi
Jan 1, 1990·International Journal of Immunopathology and Pharmacology·R MancinelliTiziana Pietrangelo
Mar 20, 2010·Free Radical Research·Provvidenza M AbruzzoMarina Marini
Feb 3, 2021·International Journal of Molecular Sciences·Caterina BernacchioniChiara Donati
Jul 3, 2021·International Journal of Molecular Sciences·Feliciano ProtasiSimona Boncompagni
Jul 5, 2021·Facial Plastic Surgery Clinics of North America·Simeon C DaeschlerGregory H Borschel
Aug 28, 2021·International Journal of Molecular Sciences·Alessia Di FonsoFeliciano Protasi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.