A subset of human gliomas shows over-expression of KIT without its amplification

Gene
Masum SainiSher Ali

Abstract

Receptor tyrosine kinase (RTK) encoded by proto-oncogene KIT is known to be involved in different types of cancers. Reportedly, KIT expression has been associated with higher grade of gliomas. Initial RT-PCR based KIT expression observed in low grade glioma cases evoked our interest to ascertain its status in glioma patients who underwent resection during 2008-2009. Contrary to earlier reports, over-expression of the RTK was observed in 32.5% glioma cases across low/high grades (n=40). Using quantitative PCR (qPCR), an up-regulation of the receptor (KIT) and its ligand (KITLG) was detected in most of the immunopositive cases at the transcript level. Sequence analysis of KIT showed two nucleotide substitutions in exons 10 and 17, in 4 and 2 cases, respectively though their pathological significance remained unclear. qPCR detected gene amplification in 2/13 glioma and allele loss in 1/13 glioma cases. This was in accordance with FISH results of these KIT positive neoplastic tissues. The data suggest that deranged expression of KIT is independent of gene amplification (p>0.05). Aberrant KIT expression is significantly associated with transcriptional up-regulation (p<0.001), though the precise mechanism(s) for transcriptional activ...Continue Reading

References

Jul 1, 1991·The Journal of Experimental Medicine·M OgawaS Nishikawa
Dec 1, 1993·Journal of Neuroscience Research·J A IdaR D McKinnon
Feb 1, 1994·Pigment Cell Research·G ScottC Abboud
Nov 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A LammieC Cordon-Cardo
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 21, 2003·American Journal of Clinical Pathology·David R LucasMichael J Mott
Dec 4, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Michael C HeinrichJonathan A Fletcher
Mar 12, 2004·Cancer Letters·Manfred Schwab
Nov 17, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Harri SihtoHeikki Joensuu
Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
May 5, 2005·Biochimica Et Biophysica Acta·Séverine TaboneJean-François Emile
Sep 1, 2005·Journal of Neuro-oncology·Neslihan CetinMurat Gokden
May 26, 2006·Nature·Judith S Sebolt-Leopold, Jessie M English
Dec 6, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elena TamboriniSilvana Pilotti
Dec 23, 2006·Science·Chava Kimchi-SarfatyMichael M Gottesman
Dec 26, 2006·Molecular Cancer Research : MCR·Marjut PuputtiNina N Nupponen
Feb 13, 2007·The Journal of Pathology·H SihtoH Joensuu
Mar 10, 2007·Current Medicinal Chemistry·A E RoussidisN K Karamanos
Oct 30, 2007·Journal of Clinical Pathology·Chris JonesKathy Pritchard-Jones
May 29, 2008·International Journal of Cancer. Journal International Du Cancer·Tea BlomNina N Nupponen
Jun 3, 2009·Scientific American·J V Chamary, Laurence D Hurst
Sep 10, 2009·Cancer Genetics and Cytogenetics·Macoura GadjiRégen Drouin
Oct 1, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Evangelia RazisGeorge Fountzilas

❮ Previous
Next ❯

Citations

Nov 23, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sunayana DagarSivaram V S Mylavarapu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.