A Suggested Diagnostic Approach for Sporadic Anthrax in Cattle to Protect Public Health.

Microorganisms
Jana AvberšekMateja Pate

Abstract

The repeated occurrence of anthrax in grazing animals should be a reminder of a widespread presence of Bacillus anthracis spores in the environment. Its rapid diagnosis is critical to protect public health. Here, we report a case of anthrax in cattle that was investigated using conventional and molecular methods. In 2015, six cows suddenly died within three days and the number of dead animals increased to a total of 12 within two weeks. At necropsy, anthrax was suspected. Therefore, spleen tissue samples were collected (from 6/12 animals) and laboratory tests (microscopy, cultivation, and real-time PCR) performed. The results of tissue staining for microscopy and cultivation were in congruence, while B. anthracis real-time PCR outperformed both. Spleen tissues from all six animals were real-time PCR-positive, while B. anthracis was successfully cultivated and detected by microscopy from the spleen of only three animals. Additionally, the ear tissue from another (1/12) cow tested positive by real-time PCR, supporting the suitability of ear clippings for molecular confirmation of B. anthracis. Genotyping of the isolates using multiple-locus variable-number tandem repeat analysis (MLVA) revealed a common source of infection as all...Continue Reading

References

Sep 1, 1966·Journal of Bacteriology·R F Knisely
Aug 24, 2006·Journal of Food Protection·Margaret A JuergensmeyerElon W Frampton
Jan 30, 2013·Journal of Microbiological Methods·M P OwenW Beyer
Feb 14, 2020·Journal of Applied Microbiology·A RohdeO Kaspari
Sep 15, 2020·PLoS Neglected Tropical Diseases·Olubunmi R AminuTaya L Forde

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