A sulfated, phosphorylated 7 kDa secreted peptide characterized by direct analysis of cell culture media

Journal of Proteome Research
Steven W TaylorSoumitra S Ghosh

Abstract

An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels directly from cell culture media in contrast to previous reports that required extensive purification and proteolytic digestion. The sulfation site was not previously described nor predicted by informatic analysis of the peptide's precursor sequence.

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Citations

Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Jong-Seo KimHie-Joon Kim
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Marina Edelson-AverbukhWolf D Lehmann
Jan 19, 2010·Molecular & Cellular Proteomics : MCP·Svetlana E NikoulinaSteven W Taylor
Jan 31, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuh-Shyong YangYi-Chih Mao
Nov 10, 2020·Mass Spectrometry Reviews·Kellen DeLaneyLingjun Li

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