A sulfhydryl oxidase from chicken egg white.

The Journal of Biological Chemistry
Karen L HooberC Thorpe

Abstract

A dimeric glycoprotein containing one FAD per approximately 80,000 Mr subunit has been isolated from chicken egg white and found to have sulfhydryl oxidase activity with a range of small molecular weight thiols. Dithiothreitol was the best substrate of those tested, with a turnover number of 1030/min, a Km of 150 microM, and a pH optimum of about 7.5. Oxidation of thiol substrates generates hydrogen peroxide in aerobic solution. Anaerobically, the ferricenium ion is a facile alternative electron acceptor. Reduction of the oxidase with dithionite or dithiothreitol under anaerobic conditions yields a two-electron intermediate (EH2) showing a charge transfer band (lambdamax 560 nm; epsilonobs 2.5 mM-1 cm-1). Complete bleaching of the flavin and discharge of the charge transfer complex require a total of four electrons. Borohydride and catalytic photoreduction give the same spectral changes. EH2, but not the oxidized enzyme, is inactivated by iodoacetamide with alkylation of 2.7 cysteine residues/subunit. These data indicate that the oxidase contains a redox-active disulfide bridge generating a thiolate to oxidized flavin charge transfer complex at the EH2 level. Sulfite treatment does not form the expected flavin adduct with the n...Continue Reading

References

May 15, 1992·Archives of Biochemistry and Biophysics·H B WhiteW W Saylor
May 1, 1990·Analytical Biochemistry·T C LehmanC Thorpe
Sep 1, 1988·Archives of Biochemistry and Biophysics·D A ClareH R Horton
Jan 1, 1988·Annual Review of Nutrition·H B White, A H Merrill
Nov 17, 1987·Biochemistry·R S de la Motte, F W Wagner
Jan 1, 1987·Methods in Enzymology·L A Goldsmith
Oct 1, 1987·Archives of Biochemistry and Biophysics·V G Janolino, H E Swaisgood
May 1, 1973·Analytical Biochemistry·E J Faeder, L M Siegel
Jul 16, 1982·Biochemical and Biophysical Research Communications·C H SchmelzerH R Horton
Jun 25, 1984·Biochimica Et Biophysica Acta·L H LashS Orrenius
Aug 2, 1983·Biochemistry·Z Jiang, C Thorpe
May 15, 1984·The Biochemical Journal·M X SliwkowskiH R Horton
Jun 1, 1980·Biochemical Society Transactions·V Massey, P Hemmerich
Jan 1, 1995·Methods in Enzymology·H F Gilbert
Jan 1, 1995·Methods in Enzymology·J H Waite

❮ Previous
Next ❯

Citations

Jul 7, 2011·Applied Microbiology and Biotechnology·Greta FaccioMarkku Saloheimo
Aug 15, 2002·Archives of Biochemistry and Biophysics·Colin ThorpeDonald L Coppock
May 16, 2012·Biochemistry·Jennifer A CoddingColin Thorpe
Feb 15, 2007·Biochemistry·Wenzhong WangColin Thorpe
Oct 20, 2007·Biochemistry·Jennifer JajeColin Thorpe
Jan 10, 2012·Journal of the American Chemical Society·Lucia BanciKostas Tokatlidis
Sep 22, 2006·Antioxidants & Redox Signaling·Agnes GörlachThomas Kietzmann
May 9, 2006·Antioxidants & Redox Signaling·Donald L Coppock, Colin Thorpe
Jun 15, 2006·Antioxidants & Redox Signaling·Carolyn S Sevier, Chris A Kaiser
May 30, 2009·Antioxidants & Redox Signaling·Feras Hatahet, Lloyd W Ruddock
Feb 9, 2010·Antioxidants & Redox Signaling·Vamsi K Kodali, Colin Thorpe
Feb 9, 2010·Antioxidants & Redox Signaling·Motti Hakim, Deborah Fass
Mar 11, 2010·Antioxidants & Redox Signaling·Dionisia P Sideris, Kostas Tokatlidis
Apr 12, 2011·PloS One·Vamsi K KodaliColin Thorpe
Jan 30, 2014·PloS One·Laura PoilletGilles Despouy
Jan 16, 2014·Food & Function·Benjamin ZeebJochen Weiss
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Einav GrossDeborah Fass
Dec 24, 2013·Antioxidants & Redox Signaling·Douglas F Lake, Douglas O Faigel
Nov 24, 2007·Journal of Molecular Histology·Kelly F PortesLia S Nakao
Aug 30, 2005·Critical Reviews in Biochemistry and Molecular Biology·Eelco van Anken, Ineke Braakman
Jun 30, 2006·Biochimica Et Biophysica Acta·Jean RadomMichèle Jouvenot
Aug 31, 2002·Analytical Biochemistry·Sonali RajeColin Thorpe
Jan 29, 2014·Free Radical Biology & Medicine·Benjamin A IsraelColin Thorpe
Jan 1, 2010·Annual Review of Food Science and Technology·Johanna BuchertKristiina Kruus
Nov 6, 2007·Biochimica Et Biophysica Acta·Erin J HecklerColin Thorpe
Nov 5, 2002·Nature Reviews. Molecular Cell Biology·Carolyn S Sevier, Chris A Kaiser
Mar 30, 2016·Annual Review of Chemical and Biomolecular Engineering·Michael C Yi, Chaitan Khosla
Jul 13, 2017·Chemical Reviews·Deborah Fass, Colin Thorpe

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.