A Sulfoxide-Based Isobaric Labelling Reagent for Accurate Quantitative Mass Spectrometry

Angewandte Chemie
Michael StadlmeierThomas Carell

Abstract

Modern proteomics requires reagents for exact quantification of peptides in complex mixtures. Peptide labelling is most typically achieved with isobaric tags that consist of a balancer and a reporter part that separate in the gas phase. An ingenious distribution of stable isotopes provides multiple reagents with identical molecular weight but a different mass of the reporter groups, allowing relative quantification of multiple samples in one measurement. Here we report a new isobaric labelling reagent, where the balancer and the reporter are linked by a sulfoxide group, which, based on the sulfoxide pyrolysis, leads to easy and asymmetric cleavage at low fragmentation energy. The fragmentation of our new design is significantly improved, yielding more intense complementary ion signals, allowing complementary ion cluster analysis as well.

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Citations

Jan 5, 2019·Chembiochem : a European Journal of Chemical Biology·Nishant PappireddiMartin Wühr
Feb 19, 2020·Expert Review of Proteomics·Loïc Dayon, Michael Affolter
Jun 20, 2018·Nature Methods·Sebastian Virreira WinterFelix Meissner
Mar 25, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Camille Lombard-Banek, John E Schiel
Jun 7, 2021·Mass Spectrometry Reviews·Xiaobo TianRainer Bischoff
Sep 18, 2018·Journal of Proteome Research·Christian D KelstrupJesper V Olsen
Dec 21, 2021·Journal of the American Society for Mass Spectrometry·Zixiang FangSaiful M Chowdhury

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