PMID: 6167554Mar 1, 1981Paper

A supertypic HLA-DR specificity (DR4+5) defined by murine monoclonal antibody

Human Immunology
J A HansenE D Thomas

Abstract

The hybridoma technique was used to produce a monoclonal antibody specific for a polymorphic determinant on human la antigens. BALB/c mice were immunized with a B-lymphoblastoid cell line derived from an HLA-DR3/DR4 positive donor. Hybridoma culture supernates were screened in two stages by means of the microcytotoxicity assay. Supernates were first tested against the la-positive immunizing cell line and against la-negative cells. Cultures identified as producing antibody cytoxic for the immunizing cell line but not for la-negative cells were subcultured and the supernates then tested against a selected panel of HLA-D homozygous cell lines (HCL). In this manner, it was possible to identify a microwell of hybrid cells producing an antibody that reacted with a polymorphic HLA-DR determinant. Cells from this culture, designated 17.15, were cloned twice by limiting dilution. Specificity was evaluated with a panel of 39 HCL and B cells from 70 normal donors. Antibody 17.15 recognized a supertypic DR "4 + 5" specificity, which was also present on DRw9 and some DR7 positive cells. These findings suggest that the cross-reactivity groups DR "4 + 5" (MB-3) and DR "4 + 7" (MT-3) defined by alloantisera may share a common supertypic specif...Continue Reading

References

Dec 1, 1978·The Journal of Experimental Medicine·R TosiD Pressman
Jan 1, 1979·Immunological Reviews·M M TruccoR Ceppellini
Jan 1, 1979·Immunological Reviews·F M BrodskyW F Bodmer
Jun 22, 1978·Nature·M M TruccoR Caeppellini

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Citations

Feb 1, 1983·Human Immunology·A ZeeviR J Duquesnoy
Mar 1, 1983·Human Immunology·C H ThompsonI F McKenzie
Jun 1, 1987·Human Immunology·T NakayamaA Wakisaka
Jan 1, 1988·Human Immunology·E M MickelsonJ A Hansen
Aug 1, 1989·Human Immunology·S F RadkaJ V Johnston
Jan 1, 1991·Human Immunology·E M MickelsonJ A Hansen
Mar 1, 1989·The Journal of Clinical Investigation·M J SheehyB S Nepom
Jan 1, 1984·Critical Reviews in Clinical Laboratory Sciences·C M Zmijewski

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