A suppressive role of p125FAK protein tyrosine kinase in hydrogen peroxide-induced apoptosis of T98G cells

Biochemical and Biophysical Research Communications
Y SonodaS Watanabe

Abstract

Protein tyrosine phosphorylation was examined on a human glioblastoma cell line, T98G, after exposure to oxidative stress in vitro. Hydrogen peroxide (1 mM) markedly induced tyrosine phosphorylation of a 125 kDa protein at 30 min after stimulation. The 125-kDa molecule phosphorylated was revealed to be a focal adhesion kinase (FAK). Tyrosine phosphorylation of p125FAK continued at least up to 5 h, and decreased after 8 h concomitant with apoptosis. Tyrosine phosphorylation of p125FAK was blocked by herbimycin A, a potent inhibitor of protein tyrosine kinases, while apoptosis was accelerated. When T98G cells were incubated with FAK antisense oligonucleotide, apoptosis was also accelerated. These results suggest that tyrosine phosphorylation of p125FAK plays a suppressive role in hydrogen peroxide-induced apoptosis.

References

Dec 1, 1992·The Journal of Clinical Investigation·N Ueda, S V Shah
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L J KornbergR L Juliano
Oct 22, 1993·Cell·D M HockenberyS J Korsmeyer
Mar 1, 1994·Molecular and Cellular Biology·M D SchallerJ T Parsons
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·H SabeH Hanafusa
Jan 1, 1994·Annual Review of Immunology·P A Baeuerle, T Henkel
Aug 1, 1996·The Journal of Cell Biology·S M FrischP Y Chan-Hui

❮ Previous
Next ❯

Citations

Sep 7, 2002·Antioxidants & Redox Signaling·Tadashi KasaharaYoshiko Sonoda
May 14, 2005·Antioxidants & Redox Signaling·Paola Chiarugi, Elisa Giannoni
Nov 23, 2006·Antioxidants & Redox Signaling·Paola Chiarugi, Francesca Buricchi
Nov 2, 2004·Biological & Pharmaceutical Bulletin·Ryoko UtsuboTadashi Kasahara
Jul 11, 2007·The Journal of Cell Biology·Yohei NiikuraKatsumi Kitagawa
Mar 12, 2015·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tadashi Kasahara
Jun 9, 2010·The Journal of Pathology·Yoshikiyo AkasakaToshiharu Ishii
Sep 26, 2006·Biochemical Pharmacology·Maroesja J van Nimwegen, Bob van de Water
Jul 9, 2004·Biochimica Et Biophysica Acta·Yoshiko SonodaTadashi Kasahara
Sep 25, 2007·Chemico-biological Interactions·Md Firoz MianEuikyung Kim
Nov 26, 2005·Pathology, Research and Practice·Jeremias WohlschlaegerHideo A Baba
Jun 11, 2002·Biochemical and Biophysical Research Communications·Shinya SakuraiTadashi Kasahara
Oct 16, 2014·Pharmacology & Therapeutics·Brian Y LeeRoger J Daly
Aug 17, 2016·Oxidative Medicine and Cellular Longevity·Meriem H Ben-MahdiCatherine Pasquier
Sep 4, 2004·The American Journal of Chinese Medicine·Yan-Hua LuDong-Zhi Wei
Oct 2, 2017·Molecular Neurobiology·Annielle Mendes Brito da SilvaManoel Arcisio-Miranda
Jun 6, 2003·The Cancer Journal·Meera NatarajanCandece L Gladson
Dec 22, 1999·Oncogene·K MaungD C Bennett
Jun 2, 2000·The Journal of Biological Chemistry·M SattlerJ D Griffin
May 12, 2018·Molecular Medicine Reports·Yanfei Cui, Shengya Yang
Jun 1, 2005·The Journal of Biological Chemistry·Wei AixinjueluoKoichi Furukawa
Jun 4, 1999·Progress in Biophysics and Molecular Biology·D D SchlaepferD J Sieg
Jan 12, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·John HuangJun Ling
Mar 4, 2000·Free Radical Biology & Medicine·R G Allen, M Tresini
Apr 6, 2005·Biochemical and Biophysical Research Communications·Konstadinos MoissogluIrwin H Gelman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis