A surface charge dependent enhanced Th1 antigen-specific immune response in lymph nodes by transfersome-based nanovaccine-loaded dissolving microneedle-assisted transdermal immunization

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Xuanjin WuMing Kong

Abstract

The efficient delivery of vaccines to draining lymph nodes and the induction of robust local immune responses are crucial for immunotherapy. Transdermal administration has been evidenced to facilitate the delivery of ingredients to lymph nodes. In this study, transfersomes with opposite surface charges were applied for antigen encapsulation and these were integrated with dissolving microneedles to investigate their effects on immune responses via transdermal immunization. The microneedles were easily inserted into mouse skin and achieved the local release of nanovaccines into the dermis through dissolution. Although anionic nanovaccines promoted cellular uptake via DC2.4, cationic nanovaccines exhibited stronger escape capacities from endocytic compartments, facilitating antigen processing via an MHC-I presentation pathway, and formed larger accumulations in lymph nodes. Compared with their anionic counterparts, the cationic nanovaccines more efficiently activated DC maturation and induced Th1 immunity; this was suggested by the significantly increased IgG2a/IgG1 ratio and elevated cytokine secretion from Th1 cells, without an enhancement in the Th2 response. Such an enhanced Th1 antigen-specific immune response in lymph nodes ...Continue Reading

References

Feb 1, 1988·Immunological Reviews·C M SnapperW E Paul
Jan 16, 1986·Biochimica Et Biophysica Acta·E Yoshihara, T Nakae
May 28, 1981·Nature·C S HenneyS Gillis
Mar 1, 1996·Immunology Today·T R Mosmann, S Sad
Sep 2, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·T NakanishiT Mayumi
Mar 17, 2001·Microbes and Infection·X Ma
Jan 24, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Xuanmao JiaoJames Wai-Kuo Shih
Apr 2, 2004·The Journal of Clinical Investigation·Igor M BelyakovJay A Berzofsky
Sep 2, 2004·Nature Medicine·Steven A RosenbergNicholas P Restifo
Jul 13, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hongtao LvJie Yan
Aug 15, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Olga BorgesHans E Junginger
Oct 13, 2007·Expert Review of Vaccines·Dennis ChristensenElse Marie Agger
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hélène Jusforgues-SaklaniMatthew L Albert
Sep 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Martin LundqvistKenneth A Dawson
Jun 30, 2011·Advanced Drug Delivery Reviews·Shuang CaiM Laird Forrest
Aug 28, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amit KumarZhengrong Cui
Oct 15, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Shu LiuAkira Yamamoto
Dec 31, 2014·Proceedings of the National Academy of Sciences of the United States of America·Catherine A FromenJoseph M DeSimone
Apr 24, 2015·BMJ : British Medical Journal·Lisa H Butterfield
Jul 7, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Peter C SoemaGideon F Kersten
Dec 15, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lea LowenfeldBrian J Czerniecki
Apr 22, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joakim KennedyRyan F Donnelly
Jun 15, 2017·Trends in Immunology·Abhishek D GargPatrizia Agostinis
Jul 6, 2017·Nature·Patrick A OttCatherine J Wu
Sep 26, 2017·Journal of Controlled Release : Official Journal of the Controlled Release Society·Guangsheng DuJuha Mönkäre
Apr 26, 2018·Nano Letters·Dongdong QiaoYongming Chen
Oct 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaoyu BaiXiguang Chen

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Citations

Dec 12, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yujie ZhangYifeng Lei
Jan 6, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Andi Dian PermanaRyan F Donnelly
Jan 10, 2021·Journal of Clinical Medicine·Vahid AlimardaniSamad Ahadian
Dec 29, 2020·Biomaterials·David WibowoBernd H A Rehm
Nov 5, 2019·International Journal of Pharmaceutics·Raquel Fernández-GarcíaDolores R Serrano
Mar 2, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Zhuoxuan LuLiming Zhang
Feb 24, 2021·International Journal of Biological Macromolecules·Adelijiang WusimanDeyun Wang
Apr 9, 2020·Acta Biomaterialia·Prateek BhardwajRinti Banerjee
Aug 6, 2020·Current Drug Delivery·Ritika Gupta, Amrish Kumar

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Methods Mentioned

BETA
transmission electron microscopy
flow cytometry
ELISA

Software Mentioned

Flow Jo

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