PMID: 9537355Apr 16, 1998Paper

A surface-exposed region of a novel outer membrane protein (P66) of Borrelia spp. is variable in size and sequence

Journal of Bacteriology
J BunikisA G Barbour

Abstract

A model of the 66-kDa outer membrane protein (P66) of Lyme disease Borrelia spp. predicts a surface-exposed loop near the C terminus. This region contains an antigen commonly recognized by sera from Lyme disease patients. In the present study, this region of P66 and homologous proteins of other Borrelia spp. were further investigated by using monoclonal antibodies, epitope mapping of P66 of Borrelia burgdorferi, and DNA sequencing. A monoclonal antibody specific for B. burgdorferi bound to the portion of P66 that was accessible to proteolysis in situ. The linear epitope for the antibody was mapped within a variable segment of the surface-exposed region. To further study this protein, the complete gene of Borrelia hermsii for a protein homologous to P66 was cloned. The deduced protein was 589 amino acids in length and 58% identical to P66 of B. burgdorferi. The B. hermsii P66 protein was predicted to have a surface-exposed region in the same location as that of B. burgdorferi's P66 protein. With primers designed on the basis of conserved sequences and PCR, we identified and cloned the same regions of P66 proteins of Borrelia turicatae, Borrelia parkeri, Borrelia coriaceae, and Borrelia anserina. The deduced protein sequences fro...Continue Reading

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Citations

Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jean I TsaoAlan G Barbour
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Evelyn RossmannReinhard Wallich
Jan 7, 2015·Molecular Microbiology·Hui ZhiJon T Skare
Aug 23, 2011·Journal of Bacteriology·Ozan S KumruWolfram R Zückert
Jan 13, 2006·Folia Parasitologica·Libor GrubhofferJames H Oliver
Sep 16, 2015·Molecular Microbiology·James L ColemanJorge L Benach
Oct 23, 2004·Emerging Infectious Diseases·Jonas BunikisAlan G Barbour
Nov 17, 2009·Nucleic Acids Research·Melisa S MedranoJenifer Coburn
Aug 23, 2005·Infection and Immunity·Alan G BarbourJonas Bunikis

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