A surfactant copolymer facilitates functional recovery of heat-denatured lysozyme

Annals of the New York Academy of Sciences
Alexandra M WalshRaphael C Lee

Abstract

The triblock copolymer poloxamer 188 is a non-cytotoxic, nonionic surfactant with both hydrophobic and hydrophilic domains. We show that P188 is able to facilitate the recovery of catalytic activity of heat-denatured lysozyme in dilute solution at low molar ratios of P188:enzyme. Heat-denatured enzyme retained 55% of native activity. After treatment with P188, the enzyme's activity was 85% of native. Because of the low molar ratios used and the non-cytotoxic nature of the compound, P188 may be of potential use in burn therapy.

References

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Citations

Jun 21, 2006·Annals of Biomedical Engineering·Raphael C LeeP Thiyagarajan
Jul 30, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Jia-Yu WangKa Yee C Lee
Jul 25, 2008·The Journal of Craniofacial Surgery·Neil D Dalal, Raphael C Lee
Nov 15, 2011·Burns : Journal of the International Society for Burn Injuries·Shi YuhuaSun Tongzhu
Oct 24, 2007·Biochimica Et Biophysica Acta·Devkumar MustafiRaphael C Lee
Sep 13, 2016·Macromolecular Bioscience·Michael J PoellmannRaphael C Lee

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