PMID: 11923271Mar 30, 2002Paper

A survey of molecular expression by photoreceptors after experimental retinal detachment

Investigative Ophthalmology & Visual Science
T S RexSteven K Fisher

Abstract

To describe changes in the localization patterns and levels of rod and cone photoreceptor proteins after experimental retinal detachment (RD). Cat retinas were detached for 1, 3, 7, or 28 days, at which time the eyecups were placed in fixative for immunocytochemical analysis or homogenized for biochemistry. Immunocytochemistry was performed using 19 probes for molecules known to be associated with photoreceptors. Protein concentrations were determined using enzyme-linked immunosorbent assay or Western blot analysis. Cone cell death was analyzed by double labeling with TdT-dUTP terminal nick-end labeling and cone-specific antibodies. Although some cones died, many survived long-term RD. Although their profiles may have changed, rod photoreceptors continued to express most of the molecules studied as long as they were alive. In contrast, the cones failed to label with almost all probes specific to them after 3 to 7 days of detachment. The exception was phosducin, which localized to both rods and cones and, in 28-day detachments, increased to 180% of the amount in normal retina. Rods and cones respond differently to RD. This difference may account for a faster return of rod vision and for the lingering changes in color vision and ...Continue Reading

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