A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans

Molecular Genetics and Genomics : MGG
Makedonka MitrevaJames P McCarter

Abstract

Plant-parasitic nematodes are important and cosmopolitan pathogens of crops. Here, we describe the generation and analysis of 1928 expressed sequence tags (ESTs) of a splice-leader 1 (SL1) library from mixed life stages of the root-lesion nematode Pratylenchus penetrans. The ESTs were grouped into 420 clusters and classified by function using the Gene Ontology (GO) hierarchy and the Kyoto KEGG database. Approximately 80% of all translated clusters show homology to Caenorhabditis elegans proteins, and 37% of the C. elegans gene homologs had confirmed phenotypes as assessed by RNA interference tests. Use of an SL1-PCR approach, while ensuring the cloning of the 5' ends of mRNAs, has demonstrated bias toward short transcripts. Putative nematode-specific and Pratylenchus -specific genes were identified, and their implications for nematode control strategies are discussed.

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Citations

Apr 4, 2008·Molecular Genetics and Genomics : MGG·Joachim JacobGodelieve Gheysen
Mar 20, 2009·BMC Genomics·Yong YinMakedonka Mitreva
Feb 3, 2009·BMC Genomics·Axel A EllingThomas J Baum
Dec 29, 2005·Parasitology Research·Bartel VanholmeGodelieve Gheysen
Nov 9, 2011·Gene·Annelies HaegemanGodelieve Gheysen
Apr 26, 2011·Molecular and Biochemical Parasitology·Annelies HaegemanGodelieve Gheysen
Apr 7, 2009·Veterinary Parasitology·Sahar AbubuckerMakedonka Mitreva
Jul 3, 2013·Molecular Plant Pathology·John T JonesRoland N Perry
May 16, 2006·International Journal for Parasitology·Yong YinMakedonka Mitreva
May 24, 2005·Molecular and Biochemical Parasitology·Fiona J ThompsonMark E Viney
Mar 30, 2010·Molecular and Biochemical Parasitology·Martin J WubbenBrian S Scheffler
Jun 15, 2016·Annual Review of Phytopathology·John Fosu-Nyarko, Michael G K Jones
Feb 10, 2018·Molecular Plant Pathology·Paulo VieiraKathryn Kamo
Aug 16, 2005·Trends in Genetics : TIG·Makedonka MitrevaJames P McCarter
Apr 13, 2021·Journal of Nematology·Kanan Saikai, Ann E MacGuidwin

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