A survey on Mycobacterium ulcerans in Mosquitoes and March flies captured from endemic areas of Northern Queensland, Australia

PLoS Neglected Tropical Diseases
Avishek SinghScott A Ritchie

Abstract

Mycobacterium ulcerans is the causative agent of Buruli ulcer (BU). This nontuberculous mycobacterial infection has been reported in 34 countries worldwide. In Australia, the majority of cases of BU have been recorded in coastal Victoria and the Mossman-Daintree areas of north Queensland. Mosquitoes have been postulated as a vector of M. ulcerans in Victoria, however the specific mode of transmission of this disease is still far from being well understood. In the current study, we trapped and analysed 16,900 (allocated to 845 pools) mosquitoes and 296 March flies from the endemic areas of north Queensland to examine for the presence of M. ulcerans DNA by polymerase chain reaction. Seven of 845 pools of mosquitoes were positive on screening using the IS2404 PCR target (maximum likelihood estimate 0.4/1,000). M. ulcerans DNA was detected from one pool of mosquitoes from which all three PCR targets: IS2404, IS2606 and the ketoreductase B domain of mycolactone polyketide synthase gene were detected. None of the March fly samples were positive for the presence of M. ulcerans DNA.

References

Apr 1, 1975·Australian and New Zealand Journal of Medicine·A J Radford
Mar 1, 1995·The American Journal of Tropical Medicine and Hygiene·B J MarstonR C Good
Aug 30, 2002·Applied and Environmental Microbiology·Laurent MarsollierBernard Carbonnelle
Aug 9, 2006·The Medical Journal of Australia·Glenn FrancisMarion Woods
Jan 26, 2008·Emerging Infectious Diseases·Paul D R JohnsonJanet A M Fyfe
Jan 26, 2008·Emerging Infectious Diseases·Tricia Y J QuekPaul D R Johnson
Mar 28, 2008·PLoS Neglected Tropical Diseases·Heather R WilliamsonPamela L C Small
Aug 3, 2010·Applied and Environmental Microbiology·John R WallacePamela L C Small
Aug 14, 2010·PLoS Neglected Tropical Diseases·Janet A M FyfePaul D R Johnson
Nov 3, 2010·ANZ Journal of Surgery·Christina M SteffenWilliam J H McBride
Sep 29, 2011·PLoS Neglected Tropical Diseases·Caroline J LavenderPaul D R Johnson
Oct 8, 2013·PLoS Neglected Tropical Diseases·Jason A TrubianoPaul D R Johnson
Dec 17, 2015·Parasites & Vectors·Patchara SriwichaiJetsumon Sattabongkot
Nov 18, 2017·Emerging Infectious Diseases·Katharina RöltgenJanet Fyfe

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Citations

Aug 27, 2021·PLoS Neglected Tropical Diseases·Anthony J MuletaChris Greening

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Methods Mentioned

BETA
PCR

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