A Sustained Immune Response Supports Long-Term Antiviral Immune Priming in the Pacific Oyster, Crassostrea gigas.

MBio
Maxime LafontCaroline Montagnani

Abstract

Over the last decade, innate immune priming has been evidenced in many invertebrate phyla. If mechanistic models have been proposed, molecular studies aiming to substantiate these models have remained scarce. We reveal here the transcriptional signature associated with immune priming in the oyster Crassostrea gigas Oysters were fully protected against Ostreid herpesvirus 1 (OsHV-1), a major oyster pathogen, after priming with poly(I·C), which mimics viral double-stranded RNA. Global analysis through RNA sequencing of oyster and viral genes after immune priming and viral infection revealed that poly(I·C) induces a strong antiviral response that impairs OsHV-1 replication. Protection is based on a sustained upregulation of immune genes, notably genes involved in the interferon pathway and apoptosis, which control subsequent viral infection. This persistent antiviral alert state remains active over 4 months and supports antiviral protection in the long term. This acquired resistance mechanism reinforces the molecular foundations of the sustained response model of immune priming. It further opens the way to applications (pseudovaccination) to cope with a recurrent disease that causes dramatic economic losses in the shellfish farmin...Continue Reading

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Citations

May 5, 2020·Environmental Microbiology·Delphine Destoumieux-GarzónLuigi Vezzulli
Aug 6, 2020·Pathogens·Jacinta R AgiusKarla J Helbig
Dec 29, 2020·Developmental and Comparative Immunology·Juliette Schneider, Jean-Luc Imler
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Apr 26, 2020·Fish & Shellfish Immunology·Andrew N Robinson, Timothy J Green
Jun 7, 2021·Journal of Proteomics·Elisabetta GianazzaIngrid Miller
Aug 29, 2021·Developmental and Comparative Immunology·Cybèle Prigot-MauriceChristine Braquart-Varnier

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Datasets Mentioned

BETA
AY509253
KY242785
KY271630
AFTI00000000
FP004478
HS119070

Methods Mentioned

BETA
RNA-seq
PCR
RNAseq
electrophoresis

Software Mentioned

GraphPad Prism
RNA STAR Galaxy
GraphPad
DESeq2
Perl script
BLAST ( Basic Local Alignment Search Tool )
MeV
FastQC
Excel
R

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