A switchable yeast display/secretion system

Protein Engineering, Design & Selection : PEDS
James A Van DeventerSachdev S Sidhu

Abstract

Display technologies such as yeast and phage display offer powerful alternatives to traditional immunization-based antibody discovery, but require conversion of displayed proteins into soluble form prior to downstream characterization. Here we utilize amber suppression to implement a yeast-based switchable display/secretion system that enables the immediate production of soluble, antibody-like reagents at the end of screening efforts. Model selections in the switchable format remain efficient, and library screening in the switchable format yields renewable sources of affinity reagents exhibiting nanomolar binding affinities. These results confirm that this system provides a seamless link between display-based screening and the production and evaluation of soluble forms of candidate binding proteins. Switchable display/secretion libraries provide a cloning-free, accessible approach to affinity reagent generation.

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Citations

Aug 16, 2016·Protein Engineering, Design & Selection : PEDS·James A Van DeventerRyan L Kelly
Aug 18, 2016·Nature Communications·Mark PogsonSai T Reddy
Nov 5, 2016·ACS Applied Materials & Interfaces·Yadveer S GrewalMatt Trau
Feb 13, 2019·Protein Engineering, Design & Selection : PEDS·Zachary T BrittonYariv Mazor
Aug 18, 2020·Protein Science : a Publication of the Protein Society·Rachel HannaSachdev S Sidhu
Apr 15, 2018·Nature Communications·Alessandro AngeliniK Dane Wittrup
Dec 6, 2019·MedChemComm·Sara LincianoAlessandro Angelini
Jan 23, 2021·ACS Chemical Biology·Mariha IslamJames A Van Deventer
Feb 1, 2020·Molecular Systems Design & Engineering·K A PottsJ A Van Deventer
Aug 25, 2018·ACS Synthetic Biology·Jessica T StieglitzJames A Van Deventer
Nov 27, 2019·ACS Combinatorial Science·Eric A MillerHadley D Sikes
Nov 4, 2021·ACS Synthetic Biology·Jessica T StieglitzJames A Van Deventer

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