PMID: 3319615Dec 30, 1987Paper

A synthetic functional metabolic compartment. The role of propinquity in a linked pair of immobilized enzymes

European Journal of Biochemistry
E T Fossel, H Hoefeler

Abstract

A system was created to model the influence of microcompartments on linked enzymatic reactions. Creatine kinase and hexokinase were covalently attached to Sepharose beads. The gel could be perfused in a specially constructed chamber inside a 360-MHz NMR spectrometer at different flow rates with solutions containing various concentrations of substrates. 31P NMR studies were carried out on the linked enzymatic reaction, creatine phosphate + glucose----creatine + glucose 6-phosphate in two enzyme gels differing in only one aspect, the average distance between hexokinase and creatine kinase. At a distance on the order of 0.1 mm between the enzymes, the average bulk concentrations of substrates and products in the perfusate determined the overall function of the linked system. At an average distance of the order of 10 nm, flux through the linked pair was much higher and much less dependent on the concentration of the intermediate substrate/product ADP/ATP. Even at adenine nucleotide concentrations far below the Km of hexokinase, substantial amounts of glucose 6-phosphate were produced when the enzymes were near but not when they were distant. From saturation transfer measurements and turnover calculations, the lifetime of ATP in the...Continue Reading

References

Nov 1, 1977·Analytical Chemistry·R S SchifreenP W Carr
Jan 1, 1985·Biopolymers·E Katchalski-KatzirY I Henis
Jul 1, 1974·Archives of Biochemistry and Biophysics·R E Gots, S P Bessman
Jan 1, 1984·Proceedings of the Society for Experimental Biology and Medicine·D E Vatner, J S Ingwall
Jan 1, 1980·Methods in Enzymology·K J Laidler, P S Bunting
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S C Tu, J W Hastings
Jan 1, 1982·The American Journal of Physiology·R A MeyerT R Brown
Jul 12, 1982·Biochimica Et Biophysica Acta·J Müller, T Zwing
May 21, 1980·Journal of Theoretical Biology·S B Lee, D D Ryu

❮ Previous
Next ❯

Citations

Apr 1, 1994·Molecular and Cellular Biochemistry·R Ventura-ClapierJ A Hoerter
Aug 1, 1992·European Journal of Biochemistry·M Arrio-DupontA d'Albis
Jun 1, 1988·Bioscience Reports·M PugnièreA Previero
Oct 18, 2014·Frontiers in Physiology·Rikke BirkedalMarko Vendelin
Feb 7, 2001·American Journal of Physiology. Cell Physiology·M VendelinV Saks
Oct 1, 1991·Biochemical Medicine and Metabolic Biology·S KunjaraP McLean

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.