A synthetic hydrogel for the high-throughput study of cell-ECM interactions

Nature Communications
Andrew D RapeSanjay Kumar

Abstract

It remains extremely challenging to dissect the cooperative influence of multiple extracellular matrix (ECM) parameters on cell behaviour. This stems in part from a lack of easily deployable strategies for the combinatorial variation of matrix biochemical and biophysical properties. Here we describe a simple, high-throughput platform based on light-modulated hyaluronic acid hydrogels that enables imposition of mutually independent and spatially continuous gradients of ligand density and substrate stiffness. We validate this system by showing that it can support mechanosensitive differentiation of mesenchymal stem cells. We also use it to show that the oncogenic microRNA, miR18a, is nonlinearly regulated by matrix stiffness and fibronectin density in glioma cells. The parallelization of experiments enabled by this platform allows condensation of studies that would normally require hundreds of independent hydrogels to a single substrate. This system is a highly accessible, high-throughput technique to study the combinatorial variation of biophysical and biochemical signals in a single experimental paradigm.

References

Jul 1, 1991·Bioconjugate Chemistry·J W KuoG D Prestwich
Dec 26, 2003·Biophysical Journal·Adam EnglerDennis Discher
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
May 8, 2007·ACS Chemical Biology·Ratmir DerdaLaura L Kiessling
May 7, 2008·Biomedical Materials·Patricia Musoke-Zawedde, Molly S Shoichet
Nov 5, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David A ReardonL Burt Nabors
Oct 27, 2009·Ultrasound in Medicine & Biology·Tormod SelbekkGeirmund Unsgaard
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Mark A LaBargeMina J Bissell
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kristopher A KilianMilan Mrksich
Apr 27, 2010·Annual Review of Biomedical Engineering·Wei SunShu Chien
May 11, 2010·Cell Stem Cell·Justin D LathiaJeremy N Rich
Sep 16, 2010·Chemistry of Materials : a Publication of the American Chemical Society·Cole A DeforestKristi S Anseth
Jan 7, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Amit Pathak, Sanjay Kumar
Mar 11, 2011·Biomacromolecules·S Van VlierbergheE Schacht
Jul 16, 2011·European Cells & Materials·C S BahneyB Johnstone
Aug 16, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jose I LopezValerie M Weaver
Oct 11, 2011·Nature Methods·Samy GobaaMatthias P Lutolf
Jan 13, 2012·Molecular Biology of the Cell·Jennifer L LeightChristopher S Chen
Feb 4, 2012·Current Opinion in Cell Biology·Adrian Ranga, Matthias P Lutolf
Feb 22, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Florian RehfeldtDennis E Discher
Jan 3, 2013·Annals of Neurology·Justin D LathiaJeremy N Rich
Mar 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mikaël M MartinoJeffrey A Hubbell
Apr 12, 2013·Advanced Materials·Mikhail V TsurkanCarsten Werner

❮ Previous
Next ❯

Citations

Feb 16, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Joanna P RysTamara Alliston
Jun 11, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Ricardo Cruz-Acuña, Andrés J García
Dec 10, 2016·ACS Applied Materials & Interfaces·Sang-Hyun PyoShaochen Chen
Feb 12, 2017·Journal of Biomedical Materials Research. Part a·Tingting XiaLi Yang
Jul 6, 2017·ACS Applied Materials & Interfaces·Zhao ZhengYiyun Cheng
Jul 18, 2017·Journal of the American Chemical Society·Xiangmeng QuChunhai Fan
Jan 5, 2018·International Journal of Molecular Sciences·Ivana ManiniDaniela Cesselli
Feb 26, 2016·Molecular Biology of the Cell·Pascale MonzoMichael P Sheetz
Nov 9, 2017·Nature Reviews. Molecular Cell Biology·Kyle H Vining, David J Mooney
Jan 13, 2018·Annual Review of Biomedical Engineering·Pamela K KreegerKristyn S Masters
Feb 11, 2018·Nature Communications·Sebastián L VegaJason A Burdick
Aug 8, 2019·Angewandte Chemie·Yizhe ZhangAmy E Herr
Nov 8, 2019·Advanced Healthcare Materials·Pengrui WangShaochen Chen
Jul 11, 2020·Nature Reviews. Materials·Kayla J WolfSanjay Kumar
Jun 17, 2020·Biomedical Materials·Mariah SarwatAurelien Forget
Aug 31, 2020·Journal of Biomedical Materials Research. Part a·Danqing ZhuFan Yang
Oct 15, 2019·Annals of the New York Academy of Sciences·Mohammed M BenmassaoudSebastián L Vega
Aug 2, 2020·Breast Cancer Research : BCR·Kelsey F RuudWeimin Li
Sep 9, 2017·Future Science OA·Weikun XiaoStephanie K Seidlits
Mar 13, 2020·APL Bioengineering·Ngan F HuangSong Li
May 18, 2016·Experimental Biology and Medicine·Anna StejskalováBenjamin D Almquist
Oct 16, 2018·Frontiers in Bioengineering and Biotechnology·Ilaria Elena PalamàBarbara Cortese
Dec 1, 2019·Bulletin of Experimental Biology and Medicine·A P ToptyginaE L Semikina
Jun 30, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Eduardo de O SanguinetAdriana Bos-Mikich
Jan 30, 2020·Current Stem Cell Research & Therapy·Wang HengJohn H Barker
Feb 12, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·David A MonteiroTamara Alliston
Mar 16, 2021·Acta Biomaterialia·Mackenzie L Grubb, Steven R Caliari
Nov 7, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nazanin GhaneAmirkianoosh Kiani
Mar 22, 2021·The Journal of Biological Chemistry·Matías Arturo PibuelSilvina Laura Lompardía
Apr 27, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Cong WangJian Ji

❮ Previous
Next ❯

Methods Mentioned

BETA
atomic force microscopy
NMR
AFM
PCR

Software Mentioned

Adobe
PeakView
ImageJ
BioRad
Photoshop
MATLAB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Materials Science & Engineering. C, Materials for Biological Applications
Wen ZhaoXuejun Wen
Proceedings of the National Academy of Sciences of the United States of America
Amit Pathak, Sanjay Kumar
© 2021 Meta ULC. All rights reserved