A synthetic peptide as a novel anticryptococcal agent

Cellular Microbiology
Elio CenciAnna Vecchiarelli

Abstract

An engineered, killer decapeptide (KP) has been synthesized based on the sequence of a recombinant, single-chain anti-idiotypic antibody (KT-scFv) acting as a functional internal image of a yeast killer toxin. Killer decapeptide exerted a strong fungicidal activity against Candida albicans, which was attributed to peptide interaction with beta-glucan. As this polysaccharide is also a critical component of the cryptococcal cell wall, we wondered whether KP was also active against Cryptococcus neoformans, a human pathogen of increasing medical importance. We found that KP was able to kill both capsular and acapsular C. neoformans cells in vitro. Furthermore, KP impaired the production of specific C. neoformans virulence factors including protease and urease activity and capsule formation, rendering the fungus more susceptible to natural effector cells. In vivo treatment with KP significantly reduced fungal burden in mice with cryptococcosis and, importantly, protected the majority of immunosuppressed animals from an otherwise lethal infection. Given the relevance of cryptococcosis in immunocompromised individuals and the inability of conventional drugs to completely resolve the infection, the results of the present study indicate...Continue Reading

References

Sep 1, 1975·Archives of Internal Medicine·H A GallisD L Stickel
Jan 1, 1991·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·L PolonelliC Chezzi
Apr 2, 1990·Carbohydrate Research·P G JamesE Reiss
Nov 1, 1985·The Journal of Infectious Diseases·S M Levitz, R D Diamond
Feb 1, 1987·Journal of Clinical Microbiology·L Polonelli, G Morace
Mar 1, 1988·Journal of Clinical Microbiology·L Polonelli, G Morace
Dec 1, 1994·Mycopathologia·S AokiV Vidotto
Jan 1, 1993·Scandinavian Journal of Immunology·L PolonelliC Chezzi
May 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·W G Powderly
Sep 1, 1996·Clinical and Diagnostic Laboratory Immunology·L C ChenA Casadevall
Mar 1, 1997·Clinical and Diagnostic Laboratory Immunology·L PolonelliJ C Cailliez
Feb 1, 1997·The Journal of Infectious Diseases·S C ChenT C Sorrell
Jul 1, 1997·Clinical Microbiology Reviews·W MaglianiL Polonelli
Oct 29, 1998·FEMS Immunology and Medical Microbiology·N SéguyJ C Cailliez
Dec 3, 1999·Microbes and Infection·D H Schmiel, V L Miller
Jan 11, 2000·Clinical Microbiology Reviews·M A Ghannoum
Jan 20, 2000·Infection and Immunity·G M CoxJ R Perfect
Feb 24, 2001·Medical Mycology·A Vecchiarelli
Sep 15, 2001·Medical Mycology·F De BernardisA Cassone
Mar 7, 2002·International Reviews of Immunology·M R OggioniL Polonelli
Aug 22, 2002·The Journal of Eukaryotic Microbiology·Dianella SavoiaLuciano Polonelli
Jan 7, 2003·Infectious Disease Clinics of North America·John R Perfect, Arturo Casadevall
May 8, 2003·Current Opinion in Infectious Diseases·Beatriz L Gómez, Joshua D Nosanchuk
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Julian R NaglikBernhard Hube
Nov 19, 2003·Molecular Microbiology·Amy J Reese, Tamara L Doering

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Citations

Oct 1, 2008·Antimicrobial Agents and Chemotherapy·Giorgio ContiLuciano Polonelli
Oct 6, 2005·Applied and Environmental Microbiology·Marcello DoniniEugenio Benvenuto
Aug 3, 2011·Future Medicinal Chemistry·Walter MaglianiLuciano Polonelli
Nov 18, 2015·Immunological Investigations·Kausik Datta, Mawieh Hamad
Jan 1, 2015·Microorganisms·Serena Muccilli, Cristina Restuccia
Jan 29, 2008·Mycological Research·Alexandre M FuentefriaPatrícia Valente
Aug 10, 2007·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·M ManfrediP Vescovi
Feb 21, 2006·Experimental Parasitology·Dianella SavoiaLuciano Polonelli
Mar 11, 2015·Journal of Peptide Science : an Official Publication of the European Peptide Society·Tecla CiociolaLuciano Polonelli
Nov 13, 2010·Microbes and Infection·Erika Seki KioshimaJosé Daniel Lopes
Sep 13, 2008·FEMS Microbiology Letters·Walter MaglianiLuciano Polonelli
Sep 2, 2009·Anais Da Academia Brasileira De Ciências·Elaine G RodriguesLuiz R Travassos
Sep 24, 2004·FEMS Yeast Research·Walter MaglianiLuciano Polonelli
Oct 26, 2005·Journal of Leukocyte Biology·Elio CenciAnna Vecchiarelli
Apr 7, 2006·Journal of Basic Microbiology·Alexandre M FuentefriaPatrícia Valente
Mar 2, 2006·The Journal of Antimicrobial Chemotherapy·Pier Luigi FioriLuciano Polonelli
Sep 9, 2008·Mycopathologia·Luiz R TravassosCarlos P Taborda
Apr 16, 2009·Molecular Pharmaceutics·Thelma A PertinhezLuciano Polonelli

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