PMID: 2108722Feb 6, 1990Paper

A synthetic peptide corresponding to human FSH beta-subunit 33-53 binds to FSH receptor, stimulates basal estradiol biosynthesis, and is a partial antagonist of FSH

Biochemistry
T A Santa ColomaL E Reichert

Abstract

We have previously shown that hFSH-beta 34-37 (KTCT) and 49-52 (TRDL) inhibit binding of 125I-hFSH to FSH receptor in calf testis membranes and that hFSH-beta 33-53, which encompasses these tetrapeptides, inhibits binding with increased potency. hFSH-beta 33-53 rapidly dimerizes under conditions utilized in the receptor binding assay (pH 7.5) so that the binding inhibition reported earlier was due to the hFSH-beta 33-53 dimer rather than the monomer. At pH 6.5, conversion to dimer does not occur, and binding inhibition could be unequivocally attributed to the monomer. Radioiodinated and alkylated hFSH-beta 33-53 binds to the FSH receptor with a Kd = (5.5 +/- 1.4) X 10(-5) M. The biological activity of hFSH-beta 33-53 was assessed by its ability to affect the conversion of androstenedione to estradiol in rat Sertoli cells cultures. FSH-beta 33-53 behaved as a partial antagonist of the FSH-induced estradiol synthesis. The required incubation medium, however, contains cystine as well as cystine, which rapidly forms a hFSH-beta Cys-(51)-S-S-Cys derivative at the pH of the incubation, 7.4. When hFSH-beta 33-53 was converted either to the hFSH-beta Cys(51)-S-S-Cys or to a carboxymethylated derivative, inhibition of FSH-induced estrad...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Aug 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R J RyanH T Keutmann
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H T KeutmannR J Ryan
Feb 1, 1987·Biology of Reproduction·J G Miller, G A Schultz
Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
Dec 7, 1984·Journal of Chromatography·B A BidlingmeyerT L Tarvin
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies

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Citations

Feb 19, 1998·Journal of Peptide Science : an Official Publication of the European Peptide Society·J C SpetzlerK Bock
May 11, 1991·Trends in Pharmacological Sciences·L E ReichertT A Santa-Coloma
Jul 11, 1991·Molecular and Cellular Endocrinology·P GrassoL E Reichert
May 1, 1992·Molecular and Cellular Endocrinology·D D VakhariaJ A Dias
Jul 1, 1992·Molecular and Cellular Endocrinology·H T Keutmann
Apr 1, 1995·Molecular and Cellular Endocrinology·K E Roth, J A Dias
May 4, 2001·Journal of Immunological Methods·M BianchiniT A Santa-Coloma
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R K CampbellW R Moyle
Oct 1, 1992·General and Comparative Endocrinology·T HayashiH Hayashi
Mar 5, 2005·Analytical Biochemistry·Panayiotis E Stevis, Darlene C Deecher
Aug 30, 1991·Biochemical and Biophysical Research Communications·J W Slootstra, E W Roubos
Jun 11, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Susha S KutteyilSmita D Mahale
May 25, 1992·Biochemical and Biophysical Research Communications·T A Santa-ColomaL E Reichert
Jan 1, 1992·Endocrine Research·S L FreemanJ C Morris
Jun 27, 2015·Cancer Immunology Research·Katarzyna UrbanskaDaniel J Powell
Jan 7, 2021·Biology of Reproduction·Kenneth L CampbellUma Vadher

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