A Synthetic Peptide CTL Vaccine Targeting Nucleocapsid Confers Protection from SARS-CoV-2 Challenge in Rhesus Macaques.

Vaccines
Paul E HarrisReid M Rubsamen

Abstract

Persistent transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has given rise to a COVID-19 pandemic. Several vaccines, conceived in 2020, that evoke protective spike antibody responses are being deployed in mass public health vaccination programs. Recent data suggests, however, that as sequence variation in the spike genome accumulates, some vaccines may lose efficacy. Using a macaque model of SARS-CoV-2 infection, we tested the efficacy of a peptide-based vaccine targeting MHC class I epitopes on the SARS-CoV-2 nucleocapsid protein. We administered biodegradable microspheres with synthetic peptides and adjuvants to rhesus macaques. Unvaccinated control and vaccinated macaques were challenged with 1 × 108 TCID50 units of SARS-CoV-2, followed by assessment of clinical symptoms and viral load, chest radiographs, and sampling of peripheral blood and bronchoalveolar lavage (BAL) fluid for downstream analysis. Vaccinated animals were free of pneumonia-like infiltrates characteristic of SARS-CoV-2 infection and presented with lower viral loads relative to controls. Gene expression in cells collected from BAL samples of vaccinated macaques revealed a unique signature associated with enhanced development of ad...Continue Reading

References

Jul 1, 1990·Statistics in Medicine·Y Hochberg, Y Benjamini
Jul 24, 2003·Scandinavian Journal of Infectious Diseases·Neelam DhimanGregory A Poland
Oct 28, 2004·Journal of Virology·Fernando AlmazánLuis Enjuanes
Jun 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kaoru Fukami-KobayashiYoshio Tateno
Dec 2, 2005·The European Respiratory Journal·S N GeorasV Casolaro
Mar 21, 2006·BMC Bioinformatics·Huynh-Hoa BuiAlessandro Sette
Jul 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·F MoscaE De Gregorio
Apr 8, 2010·The Journal of Allergy and Clinical Immunology·Anne M FitzpatrickUNKNOWN National Institutes of Health/National Heart, Lung, and Blood Institute's Severe Asthma Research Program
Mar 19, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hannah RabensteinReinhard Obst
Jan 24, 2015·Science·Mathias UhlénFredrik Pontén
May 20, 2015·Vaccine·Thorunn OlafsdottirAli M Harandi
May 14, 2016·World Journal of Virology·Muthannan Andavar Ramakrishnan
Oct 6, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vanessa JurtzMorten Nielsen
Nov 4, 2017·Science·Leah C KatzelnickEva Harris
Feb 10, 2018·Methods in Molecular Biology·Rupesh Kanchi RaviMahdieh Khosroheidari
Oct 26, 2018·Nucleic Acids Research·Randi VitaBjoern Peters
Jan 1, 2003·Chinese Science Bulletin = Kexue Tongbao·E'de QinHuanming Yang
Apr 23, 2020·Animal Models and Experimental Medicine·Pin YuChuan Qin
May 22, 2020·Science·Jingyou YuDan H Barouch
May 22, 2020·Science·Abishek ChandrashekarDan H Barouch
Jun 9, 2020·BioRxiv : the Preprint Server for Biology·Vincent J MunsterEmmie de Wit
Jun 9, 2020·BioRxiv : the Preprint Server for Biology·Neeltje van DoremalenVincent J Munster
Jul 30, 2020·The New England Journal of Medicine·Kizzmekia S CorbettBarney S Graham
Jul 31, 2020·Nature·Noe B MercadoDan H Barouch

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