A synthetic peptide derived from a COOH-terminal domain of the insulin receptor specifically enhances insulin receptor signaling.

The Journal of Biological Chemistry
H K KoleMichel Bernier

Abstract

The role of the insulin receptor COOH-terminal domain in the regulation of insulin signal transduction was explored with a variety of synthetic peptides. One of the peptides, termed peptide HC, whose structure corresponds to residues 1293-1307 of the insulin proreceptor sequence, enhanced insulin-stimulated autophosphorylation of the insulin receptor in cell-free systems and in semipermeabilized Chinese hamster ovary (CHO) cells that had been transfected with an expression plasmid encoding the human insulin receptor (CHO/HIRc) at concentrations where there was no detectable effect on basal autophosphorylation levels or on receptor dephosphorylation. A lipophilic analogue of peptide HC, stearyl peptide HC, added to intact CHO/HIRc cells enhanced significantly insulin-stimulated insulin receptor autophosphorylation while having no effect on ligand-stimulated receptor phosphorylation in CHO cells overexpressing either the IGF-1 receptor or epidermal growth factor receptor. Addition of stearyl peptide HC to CHO/HIRc cells resulted in a 2.4 +/- 0.3-fold increase in the amount of insulin-stimulated phosphatidylinositol 3-kinase detected in anti-IRS-1 immunoprecipitates and a 2.1 +/- 0.6-fold increase in the levels of tyrosine phospho...Continue Reading

References

Nov 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·M G MyersM F White
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N B RudermanL C Cantley
Feb 13, 1986·Biochemical and Biophysical Research Communications·R A Kohanski, M D Lane
Oct 14, 1994·Biochemical and Biophysical Research Communications·T R BurkeP P Roller
Feb 1, 1994·The American Journal of Physiology·J Lee, P F Pilch
Apr 15, 1994·Biochemical and Biophysical Research Communications·S Clark, N Konstantopoulos

❮ Previous
Next ❯

Citations

Sep 24, 2004·Journal of Molecular Recognition : JMR·Johannes OehlkeMichael Bienert
Aug 7, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joseph SebeoDeanna L Benson
Oct 22, 2003·The Journal of Cell Biology·Yong-Kook KwonMichel Bernier
Jul 5, 2005·Expert Opinion on Investigational Drugs·J L Evans, B Jallal
Jan 31, 2003·Expert Opinion on Investigational Drugs·Zhong-Yin Zhang, Seung-Yub Lee
Apr 17, 1998·Critical Reviews in Biochemistry and Molecular Biology·Z Y Zhang
Nov 5, 1998·Bioorganic & Medicinal Chemistry·S D TaylorZ Huang
May 8, 2000·Journal of Cellular Biochemistry·M BernierS Kole
Jun 25, 1998·Archives of Biochemistry and Biophysics·S DesmaraisC Ramachandran
Jan 24, 1998·Biochemical and Biophysical Research Communications·R LammersA Ullrich
Mar 6, 2004·Archives of Biochemistry and Biophysics·Linda GengKe-He Ruan

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.