Dec 21, 2000

A system for monitoring the response of uniaxial strain on cell seeded collagen gels

Medical Engineering & Physics
C CacouJulia C Shelton

Abstract

The success of cell seeded constructs for the repair of collagenous tissues may be improved by the use of mechanical stimulation in vitro. A mechanical loading apparatus, termed the cell straining system, was developed according to a set of design criteria, to enable cell seeded constructs to be cyclically loaded in tension. A suitable cell seeded collagen gel model system was used to characterise the apparatus. These gels were subjected to a cyclic strain of 10% superimposed on two separate tare loads of 2 and 10 mN, while being maintained in cell culture conditions. The computer controlled apparatus was shown to be capable of monitoring the individual loads on six specimens simultaneously, to an accuracy of 0.02 mN. Results indicated a wide variability between individual specimens. Following cyclic loading, the cell seeded collagen gels exhibited an increase in structural stiffness compared with the unloaded controls. This novel and versatile apparatus will provide a means of enhancing structural and mechanical integrity of tissue engineered repair systems.

  • References15
  • Citations29

References

  • References15
  • Citations29

Citations

Mentioned in this Paper

Cell Culture Techniques
Straining
Zyderm
Kinematics
Vicia sativa
Fibroblasts
Collagen Location
Clinical Engineering
Stiffness
Collagen Activity

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