A systematic approach toward comparing electrospray ionization efficiencies of derivatized and non-derivatized amino acids and biogenic amines

Journal of Mass Spectrometry : JMS
Artur GornischeffRiin Rebane

Abstract

Ionization efficiency (IE) in mass spectrometry (MS) has been studied for many different compounds, and different IE scales have been constructed in order to quantitatively characterize IE. In the case of MS, derivatization has been used to increase the sensitivity of the method and to lower the limits of detection. However, the influence of derivatization on IE across different compounds and different derivatization reagents has not been thoroughly researched, so that practitioners do not have information on the IE-enhancing abilities of different derivatization reagents. Moreover, measuring IE via direct infusion of compounds cannot be considered fully adequate. Since derivatized compounds are in complex mixtures, a chromatographic method is needed to separate these compounds to minimize potential matrix effects. In this work, an IE measurement system with a chromatographic column was developed for mainly amino acids and some biogenic amines. IE measurements with liquid chromatography electrospray ionization mass spectrometry (LC/ESI/MS) were carried out, and IE scales were constructed with a calibration curve for compounds with and without derivatization reagent diethyl ethoxymethylenemalonate. Additionally, eluent compositi...Continue Reading

References

Aug 1, 1990·International Journal of Food Microbiology·B ten BrinkJ H Huis in 't Veld
Jul 25, 2000·Analytical Chemistry·N B Cech, C G Enke
May 9, 2002·Mass Spectrometry Reviews·N B Cech, C G Enke
Jun 15, 2006·Analytical Chemistry·Hamid Mirzaei, Fred Regnier
Jan 10, 2008·Rapid Communications in Mass Spectrometry : RCM·Ivo LeitoRisto Tanner
Sep 19, 2008·Journal of Chromatography. a·Risto Kostiainen, Tiina J Kauppila
Mar 12, 2010·Analytical Chemistry·Merit OssIvo Leito
Mar 9, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yusuke IwasakiHiroyuki Nakazawa
Apr 20, 2011·Expert Review of Proteomics·Peter Liuni, Derek J Wilson
Jul 17, 2012·European Journal of Mass Spectrometry·Riin Rebane, Koit Herodes
Aug 15, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Riin RebaneKoit Herodes
Jun 1, 2013·Journal of Mass Spectrometry : JMS·Anneli KruveIvo Leito
Aug 12, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maarja-Liisa OldekopRiin Rebane
Aug 22, 2014·Journal of the American Society for Mass Spectrometry·Jaanus LiigandRodolphe Antoine
Aug 8, 2015·Journal of the American Society for Mass Spectrometry·Jaanus LiigandIvo Leito
Jan 21, 2016·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Pamela K JensenMark A McGuire
Mar 5, 2016·Analytical Chemistry·Riin RebaneIvo Leito
Jul 14, 2016·Journal of Food Science and Technology·K B BijiT K Srinivasa Gopal
Nov 11, 2016·Journal of the American Society for Mass Spectrometry·Christopher J CramerAmin M Kamel
Apr 4, 2017·Analytical Chemistry·Anneli Kruve, Karl Kaupmees
May 11, 2017·Analytical Chemistry·Piia LiigandAnneli Kruve
Sep 21, 2017·Journal of Pharmaceutical and Biomedical Analysis·Yanting SongMakoto Tsunoda

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Citations

Aug 6, 2019·Rapid Communications in Mass Spectrometry : RCM·Riin RebaneIvo Leito
Jun 14, 2019·Current Opinion in Clinical Nutrition and Metabolic Care·Mariëlle P K J EngelenNicolaas E P Deutz
Aug 7, 2021·Rapid Communications in Mass Spectrometry : RCM·Merit OssIvo Leito

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