A systematic screen of beta(2)-microglobulin and insulin for amyloid-like segments

Proceedings of the National Academy of Sciences of the United States of America
Magdalena I IvanovaDavid Eisenberg

Abstract

Identifying sequence determinants of fibril-forming proteins is crucial for understanding the processes causing >20 proteins to form pathological amyloid depositions. Our approach to identifying which sequences form amyloid-like fibrils is to screen the amyloid-forming proteins human insulin and beta(2)-microglobulin for segments that form fibrils. Our screen is of 60 sequentially overlapping peptides, 59 being six residues in length and 1 being five residues, covering every noncysteine-containing segment in these two proteins. Each peptide was characterized as amyloid-like or nonfibril-forming. Amyloid-like peptides formed fibrils visible in electron micrographs or needle-like microcrystals showing a cross-beta diffraction pattern. Eight of the 60 peptides (three from insulin and five from beta(2)-microglobulin) were identified as amyloid-like. The results of the screen were used to assess the computational method, and good agreement between prediction and experiments was found. This agreement suggests that the pair-of-sheets, zipper spine model on which the computational method is based is at least approximately correct for the structure of the fibrils and suggests the nature of the sequence signal for formation of amyloid-li...Continue Reading

References

Aug 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W E KlunkD J Abraham
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P WestermarkK H Johnson
May 1, 1997·Journal of Pharmaceutical Sciences·J BrangeE Rasmussen
Apr 15, 2000·Journal of Molecular Biology·C E MacPhee, C M Dobson
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·M BalbirnieD S Eisenberg
Mar 22, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C M Dobson
Jun 8, 2002·Journal of Molecular Biology·Jean L WhittinghamG Guy Dodson
Jul 4, 2002·The Journal of Biological Chemistry·Meital RechesEhud Gazit
Sep 7, 2002·The Journal of Biological Chemistry·Lars TjernbergJan Johansson
Nov 1, 2002·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Per WestermarkJean D Sipe
Dec 19, 2002·Journal of Molecular Biology·Susan JonesSheena E Radford
Nov 19, 2003·Biochemistry·Magdalena I IvanovaDavid Eisenberg
Dec 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Manuela López de la Paz, Luis Serrano
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Salvador VenturaLuis Serrano
Jun 10, 2005·Nature·Rebecca NelsonDavid Eisenberg
Nov 24, 2005·The FEBS Journal·Per Westermark
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael J ThompsonDavid Eisenberg

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Citations

Jan 1, 2010·Cellular and Molecular Life Sciences : CMLS·Ursula Hinz, UNKNOWN UniProt Consortium
Sep 20, 2006·Accounts of Chemical Research·David EisenbergChristian Riekel
May 9, 2012·Biomacromolecules·Raimon SabateSalvador Ventura
Oct 30, 2009·Proceedings of the National Academy of Sciences of the United States of America·Magdalena I IvanovaDavid Eisenberg
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matthew BiancalanaShohei Koide
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Lukasz GoldschmidtDavid Eisenberg
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Katarzyna DomanskaJan Steyaert
Sep 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jacques-Philippe ColletierDavid Eisenberg
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Andrej PetricJorge R Barrio
Jul 16, 2009·Protein Engineering, Design & Selection : PEDS·Poh K Teng, David Eisenberg
Feb 28, 2007·BMC Bioinformatics·Oscar Conchillo-SoléSalvador Ventura
May 3, 2007·PLoS Biology·Bente VestergaardDmitri I Svergun
Jun 23, 2011·PLoS Biology·Meytal LandauDavid Eisenberg
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Michael J ThompsonDavid Eisenberg
Mar 19, 2014·Antioxidants & Redox Signaling·Hugo FragaSalvador Ventura
May 22, 2013·Molecular Diversity·Remigiusz BąchorZbigniew Szewczuk
Mar 9, 2017·International Journal of Molecular Sciences·Ajda Taler-VerčičEva Žerovnik
Apr 28, 2009·Journal of Molecular Biology·Katy E RoutledgeSheena E Radford
Aug 7, 2009·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Niels H H Heegaard
Mar 16, 2017·Journal of Molecular Recognition : JMR·Vladyslava KovalskaSergiy Yarmoluk
Oct 30, 2014·Chemical Communications : Chem Comm·Dmitry KurouskiIgor K Lednev
Nov 5, 2011·Journal of Peptide Science : an Official Publication of the European Peptide Society·Chandrasekaran SivakamaSundariRamakrishnan Nagaraj
Mar 23, 2007·Molecular Microbiology·Anton V Zavialov, Stefan D Knight
May 1, 2007·Nature·Michael R SawayaDavid Eisenberg
Dec 13, 2007·Journal of Computational Chemistry·Oliver J Clarke, Martin J Parker
Jun 18, 2009·Chembiochem : a European Journal of Chemical Biology·Jürgen HaasHelmut Grubmüller
Aug 29, 2009·Journal of Peptide Science : an Official Publication of the European Peptide Society·Nitin ChaudharyRamakrishnan Nagaraj
Dec 23, 2009·Protein Science : a Publication of the Protein Society·Susan Tzotzos, Andrew J Doig
Mar 1, 2019·Scientific Reports·Robin Axel Ruiz-ZamoraLuis Del Pozo-Yauner
Oct 9, 2008·FEBS Letters·Alba EspargaróSalvador Ventura

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