A systematic study of the cell wall composition of Kluyveromyces lactis

Yeast
Katja BackhausJ J Heinisch

Abstract

In many ascomycetous yeasts, the cell wall is composed of two main types of macromolecules: (a) polysaccharides, with a high content of beta-1,6- and beta-1,3-linked glucan chains and minor amounts of chitin; and (b) cell wall proteins of different types. Synthesis and maintenance of these macromolecules respond to environmental changes, which are sensed by the cell wall integrity (CWI) signal transduction pathway. We here present a first systematic analysis of the cell wall composition of the milk yeast, Kluyveromyces lactis. Electron microscopic analyses revealed that exponentially growing cells of K. lactis supplied with glucose as a carbon source have a wall thickness of 64 nm, as compared to 105 nm when growing on 3% ethanol. Despite their increased wall thickness, ethanol-grown cells were more sensitive to the presence of zymolyase in the growth medium. Mass spectrometric analysis identified 22 covalently linked cell wall proteins, including 19 GPI-modified proteins and two Pir wall proteins. Importantly, the composition of the cell wall glycoproteome depended on carbon source and growth phase. Our results clearly illustrate the dynamic nature of the cell wall of K. lactis and provide a firm base for studying its regulation.

References

May 1, 1980·The Journal of Cell Biology·J MolanoE Cabib
May 1, 1993·Molecular Microbiology·J HeinischC P Hollenberg
Dec 1, 1996·The Journal of Cell Biology·V BrizzioM D Rose
Jul 31, 1998·Micron : the International Research and Review Journal for Microscopy·M Osumi
Sep 22, 1998·Fungal Genetics and Biology : FG & B·A R Hardham, H J Mitchell
Oct 9, 1998·Applied Microbiology and Biotechnology·T H NguyenP L Rogers
May 18, 1999·Journal of Molecular Biology·J J JacobyJ J Heinisch
Jul 8, 1999·Molecular & General Genetics : MGG·I GeorisJ Vandenhaute
Jun 23, 2000·Enzyme and Microbial Technology·M I González-SisoM E Cerdán
Jun 23, 2000·Enzyme and Microbial Technology·K D BreunigA M Zeeman
Aug 23, 2000·Yeast·D UccellettiC Palleschi
Feb 13, 2001·Microscopy Research and Technique·R Wright
Aug 8, 2002·FEMS Microbiology Reviews·Frans M KlisStanley Brul
Nov 9, 2002·FEMS Microbiology Letters·Karim BahmedJoël Coulon
Aug 9, 2003·Letters in Applied Microbiology·B Aguilar-Uscanga, J M François
Dec 19, 2003·Nucleic Acids Research·David ShermanUNKNOWN Génolevures Consortium
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
Nov 18, 2005·Biotechnology Advances·Marta Rubio-Texeira
Feb 24, 2006·The Journal of Biological Chemistry·Margit EckerWidmar Tanner
Apr 25, 2006·FEMS Yeast Research·Albert J J van OoyenChristopher H Taron
Jan 1, 1933·The Biochemical Journal·L A Elson, W T Morgan
Sep 2, 2006·Microbiology·Rosaura RodicioJürgen J Heinisch
Oct 27, 2006·Molecular Biology of the Cell·Hai Lan PiaoGregory S Payne
Sep 11, 2007·Fungal Genetics and Biology : FG & B·Rosaura RodicioJürgen J Heinisch
Sep 1, 2009·Microbial Cell Factories·M Isabel González-SisoM Esperanza Cerdán
Sep 11, 2009·Proteomics·Catherine L MadingerJack S Benner
Sep 22, 2009·Nature Chemical Biology·Vincent DupresYves F Dufrêne
Nov 6, 2009·Journal of Visualized Experiments : JoVE·Philippe DesjardinsMichael Allen
Jul 20, 2010·Yeast·Alice G SorgoFrans M Klis

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Citations

Oct 3, 2012·BMC Genomics·Oscar DiasIsabel Rocha
Oct 19, 2012·Journal of Cell Science·Jürgen J HeinischYves F Dufrêne
Aug 25, 2011·Future Microbiology·Frans M KlisStanley Brul
Mar 19, 2014·Bioprocess and Biosystems Engineering·Siti Fatimah Zaharah Mohamad FuziRosli Md Illias
Jun 2, 2011·European Journal of Cell Biology·Katja BackhausJürgen J Heinisch
Aug 12, 2014·Biochimica Et Biophysica Acta·Dorthe RippertJürgen J Heinisch
Jul 20, 2010·Yeast·Rosaura Rodicio, Jürgen J Heinisch
Apr 30, 2014·Biotechnology Journal·Oscar DiasIsabel Rocha
Nov 16, 2014·Applied and Environmental Microbiology·Christian KockJürgen J Heinisch
Sep 7, 2019·Current Protein & Peptide Science·Verónica PlazaLuis Castillo
Jul 31, 2019·Applied Microbiology and Biotechnology·Jesús Urbar-UlloaJorge Verdín
Dec 2, 2020·International Journal of Molecular Sciences·Renata TeparićVladimir Mrša
Feb 13, 2021·Journal of Fungi·Mateja LozančićVladimir Mrša
Jan 21, 2021·International Journal of Molecular Sciences·Ionut Avramia, Sonia Amariei
Sep 2, 2010·ACS Nano·Vincent DupresJürgen J Heinisch
Aug 27, 2021·Journal of Cell Science·Marius MusielakJürgen J Heinisch

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