A Systematic Study of the Impact of Estrogens and Selective Estrogen Receptor Modulators on Prostate Cancer Cell Proliferation.

Scientific Reports
Camille LafrontÉtienne Audet-Walsh

Abstract

The estrogen signaling pathway has been reported to modulate prostate cancer (PCa) progression through the activity of estrogen receptors α and β (ERα and ERβ). Given that selective estrogen receptor modulators (SERMs) are used to treat breast cancer, ERs have been proposed as attractive therapeutic targets in PCa. However, many inconsistencies regarding the expression of ERs and the efficacy of SERMs for PCa treatment exist, notably due to the use of ERβ antibodies lacking specificity and treatments with high SERM concentrations leading to off-target effects. To end this confusion, our objective was to study the impact of estrogenic and anti-estrogenic ligands in well-studied in vitro PCa models with appropriate controls, dosages, and ER subtype-specific antibodies. When using physiologically relevant concentrations of nine estrogenic/anti-estrogenic compounds, including five SERMs, we observed no significant modulation of PCa cell proliferation. Using RNA-seq and validated antibodies, we demonstrate that these PCa models do not express ERs. In contrast, RNA-seq from PCa samples from patients have detectable expression of ERα. Overall, our study reveals that commonly used PCa models are inappropriate to study ERs and indicate ...Continue Reading

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Citations

Feb 19, 2021·Computational and Structural Biotechnology Journal·Taoyu YeYang Zhang
Sep 21, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Namita MahadikRajkumar Banerjee

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Methods Mentioned

BETA
RNA-seq
biopsies
Assay

Software Mentioned

UCSC genome browser
FastQC
UCSC browser
XL STATS
Trimmomatic

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