A systematically chromosomally engineered Escherichia coli efficiently produces butanol

Metabolic Engineering
Hongjun DongYin Li

Abstract

Biotechnological production of butanol in heterologous hosts has recently attracted many interests. Of the heterologous hosts investigated to date, engineered Escherichia coli has shown a superior butanol yield than the natural butanol-producing clostridial strains. However, all reported butanol-producing E. coli strains contain vectors and inducible promoters, which means antibiotics and inducers are required in the fermentation. The aim of this study was to develop a completely chromosomally engineered E. coli strain capable of producing butanol efficiently in the absence of vectors, antibiotics, and inducers. The challenges are the expression strength of chromosomally engineered genes under constitutive promoters is much weaker than the vector engineered genes under inducible promoters. To address these challenges, the butanol pathway was engineered into the chromosome in the first place, then the host and the butanol pathway was iteratively engineered through rational and non-rational strategies to develop an efficient butanol producer where the heterologous butanol pathway fits the host well. Finally, a systematically chromosomally engineered E. coli strain EB243, in which 33 native genes were deleted and 5 heterologous ge...Continue Reading

Citations

Jan 27, 2019·Materials·Beata KolesinskaDorota Kregiel
Jul 29, 2020·Frontiers in Bioengineering and Biotechnology·Liya LiangCarrie A Eckert
Sep 27, 2018·Biotechnology for Biofuels·Fengxue XinMin Jiang
Oct 20, 2020·Frontiers in Bioengineering and Biotechnology·Zhiquan WangYuanyuan Ma
Apr 9, 2018·Metabolic Engineering·Xia WangShihui Yang
Sep 7, 2018·Metabolic Engineering·Heyun WuXixian Xie
May 10, 2021·Trends in Biotechnology·Li ZhuSheng Yang
Aug 1, 2020·Journal of Environmental Management·Surbhi SharmaTejraj M Aminabhavi
Oct 27, 2021·World Journal of Microbiology & Biotechnology·Qi LiSheng Yang

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