A Systems Biology Study in Tomato Fruit Reveals Correlations between the Ascorbate Pool and Genes Involved in Ribosome Biogenesis, Translation, and the Heat-Shock Response

Frontiers in Plant Science
Rebecca G StevensChristophe Rothan

Abstract

Changing the balance between ascorbate, monodehydroascorbate, and dehydroascorbate in plant cells by manipulating the activity of enzymes involved in ascorbate synthesis or recycling of oxidized and reduced forms leads to multiple phenotypes. A systems biology approach including network analysis of the transcriptome, proteome and metabolites of RNAi lines for ascorbate oxidase, monodehydroascorbate reductase and galactonolactone dehydrogenase has been carried out in orange fruit pericarp of tomato (Solanum lycopersicum). The transcriptome of the RNAi ascorbate oxidase lines is inversed compared to the monodehydroascorbate reductase and galactonolactone dehydrogenase lines. Differentially expressed genes are involved in ribosome biogenesis and translation. This transcriptome inversion is also seen in response to different stresses in Arabidopsis. The transcriptome response is not well correlated with the proteome which, with the metabolites, are correlated to the activity of the ascorbate redox enzymes-ascorbate oxidase and monodehydroascorbate reductase. Differentially accumulated proteins include metacaspase, protein disulphide isomerase, chaperone DnaK and carbonic anhydrase and the metabolites chlorogenic acid, dehydroascorb...Continue Reading

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Citations

Nov 13, 2018·The Plant Journal : for Cell and Molecular Biology·Christophe RothanMathilde Causse
Sep 27, 2019·Journal of Experimental Botany·Catalina Duran GarzonCatherine Rayon
Aug 21, 2019·Frontiers in Plant Science·Sara CiminiVittoria Locato
Oct 18, 2019·Frontiers in Plant Science·Guillaume DecrosPierre Pétriacq
Oct 23, 2020·Critical Reviews in Food Science and Nutrition·Sanskriti VatsRupesh Deshmukh

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Methods Mentioned

BETA
genetic modifications
protein folding
NMR
electrophoresis
transgenic
AsA
GTPase
RNA-seq

Software Mentioned

Solycs
TBLASTN
Samespot
R
R Development - Core - Team
ImageJ
WGCNA “ R ”
Cytoscape
WGCNA script
Genepix

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