A systems level approach to temporal expression dynamics in Drosophila reveals clusters of long term memory genes

PLoS Genetics
Julianna BozlerGiovanni Bosco

Abstract

The ability to integrate experiential information and recall it in the form of memory is observed in a wide range of taxa, and is a hallmark of highly derived nervous systems. Storage of past experiences is critical for adaptive behaviors that anticipate both adverse and positive environmental factors. The process of memory formation and consolidation involve many synchronized biological events including gene transcription, protein modification, and intracellular trafficking: However, many of these molecular mechanisms remain illusive. With Drosophila as a model system we use a nonassociative memory paradigm and a systems level approach to uncover novel transcriptional patterns. RNA sequencing of Drosophila heads during and after memory formation identified a number of novel memory genes. Tracking the dynamic expression of these genes over time revealed complex gene networks involved in long term memory. In particular, this study focuses on two functional gene clusters of signal peptides and proteases. Bioinformatics network analysis and prediction in combination with high-throughput RNA sequencing identified previously unknown memory genes, which when genetically knocked down resulted in behaviorally validated memory defects.

References

May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Y DudaiS Benzer
Jan 1, 1991·Annual Review of Biochemistry·H R Herschman
Nov 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·S H Barondes, H D Cohen
Sep 16, 1966·Science·J L McGaugh
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·C H BaileyE R Kandel
Feb 14, 1998·Nature·M S GrotewielR L Davis
Jun 5, 1999·Cellular and Molecular Life Sciences : CMLS·O Stork, H Welzl
Feb 13, 2001·The Journal of Clinical Investigation·M P Mattson, S Camandola
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·T OsterwalderH Keshishian
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·G RomanR L Davis
Dec 4, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sebastiano CavallaroDaniel L Alkon
Feb 21, 2003·Current Biology : CB·Josh DubnauTim Tully
Aug 25, 2004·Neurotoxicity Research·Lionel Muller IgazJorge H Medina
Nov 24, 2004·Biochimie·Francine LevyLaurence Ehret-Sabatier
May 21, 2005·Science·Frederic Mery, Tadeusz J Kawecki
Apr 6, 2006·Genes, Brain, and Behavior·S ParatoreS Cavallaro
Sep 21, 2007·Neuron·Elizabeth S HeckscherGraeme W Davis
Nov 15, 2007·PloS One·Savita AyyarPat Simpson
Feb 15, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Renato FrischknechtPeter Sonderegger
Sep 9, 2008·Nature Neuroscience·François V BolducTim Tully
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 21, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fabrice LagasseFrederic Mery
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
Apr 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tijana CopfThomas Preat
May 11, 2011·Cold Spring Harbor Perspectives in Biology·Anne E West, Michael E Greenberg
Nov 8, 2011·Molecular Cell·Trent FowlerAnanda L Roy
Jul 19, 2012·PLoS Genetics·Yoshinori AsoHiromu Tanimoto
Nov 23, 2012·G3 : Genes - Genomes - Genetics·Ari WinbushMichelle N Arbeitman
Jan 26, 2013·Science·Pierre-Yves Plaçais, Thomas Preat

❮ Previous
Next ❯

Citations

Oct 13, 2018·PLoS Genetics·Raquel Barajas-AzpeletaKausik Si
Nov 23, 2018·G3 : Genes - Genomes - Genetics·Balint Z KacsohGiovanni Bosco
Apr 18, 2019·Behavioral and Brain Functions : BBF·Gregory L EngelAmanda J Crocker
Jun 27, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jenifer C Kaldun, Simon G Sprecher
Nov 3, 2020·EMBO Reports·Lewis ArthurtonLuis Alberto Baena-Lopez
Feb 2, 2021·Journal of Neurogenetics·Nicholas RaunJamie M Kramer
Dec 14, 2019·Current Opinion in Immunology·Mark Austin Hanson, Bruno Lemaitre
Apr 17, 2021·Frontiers in Behavioral Neuroscience·Camilla RoselliIsaac Cervantes-Sandoval

❮ Previous
Next ❯

Methods Mentioned

BETA
gene knock down
PCR

Software Mentioned

IMP
R package pheatmap
edgeR
κTry
trimmomatic
Integrative Multi - species Prediction ( IMP )
illumina
STAR
package plotrix
DAVID

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.