A T-cell clone recognizing an MLC stimulating epitope located on the DRw11 beta 1 chain.

Human Immunology
A UrlacherM M Tongio

Abstract

An alloreactive proliferative T-cell clone, 6065 WS, was obtained in an intrafamilial cell combination where the stimulator was a homozygous DRw11, DRw52, DQw3 son and the responder his haploidentical mother. Proliferation assays on eight local DRw11 families, 75 homozygous B-cell lines (Tenth Histocompatibility Workshop panel) and blocking assays with monoclonal antibodies showed that clone 6065 WS recognizes an epitope on the DRw11 beta 1 chain. Comparison of the reactivity of clone 6065 WS with cells expressing the three known DRw11 beta 1 amino acid sequences identified two unique amino acids at positions 71 and 86 which contribute to determining the specific recognition by the T-cell clone 6065 WS. Our data suggest that one or both of these amino acids can either be directly involved in the recognition by the T-cell receptor or responsible for critical conformation of the determinants on the DR molecule. Alternatively, they could affect recognition of a self peptide bound to the major histocompatibility complex class II molecule.

References

Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B S LeeH O McDevitt
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D D EckelsJ R Lamb
Jul 1, 1985·Immunological Reviews·J TrowsdaleJ Bodmer
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G AngeliniB Mach
Jan 1, 1988·Immunogenetics·V SteimleJ T Epplen
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M TiercyB Mach
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·B MachC Ucla
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J I BellH O McDevitt
Jul 1, 1985·Immunological Reviews·A J KormanJ L Strominger
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P RolliniJ Gorski
Dec 5, 1964·Nature·P I TERASAKI, J D MCCLELLAND
Jan 1, 1988·Immunology Today·J Trowsdale, R Duncan Campbell

❮ Previous
Next ❯

Related Concepts

Related Feeds