A targeted in situ hybridization screen identifies putative seminal fluid proteins in a simultaneously hermaphroditic flatworm

BMC Evolutionary Biology
Michael WeberSteven A Ramm

Abstract

Along with sperm, in many taxa ejaculates also contain large numbers of seminal fluid proteins (SFPs). SFPs and sperm are transferred to the mating partner, where they are thought to play key roles in mediating post-mating sexual selection. They modulate the partner's behavior and physiology in ways that influence the reproductive success of both partners, thus potentially leading to sexual conflict. Despite the presumed general functional and evolutionary significance of SFPs, their identification and characterization has to date focused on just a few animal groups, predominantly insects and mammals. Moreover, until now seminal fluid profiling has mainly focused on species with separate sexes. Here we report a comprehensive screen for putative SFPs in the simultaneously hermaphroditic flatworm Macrostomum lignano. Based on existing transcriptomic data, we selected 150 transcripts known to be (a) predominantly expressed in the tail region of the worms, where the seminal fluid-producing prostate gland cells are located, and (b) differentially expressed in social environments differing in sperm competition level, strongly implying that they represent a phenotypically plastic aspect of male reproductive allocation in this species....Continue Reading

References

Dec 1, 1993·Biology of Reproduction·G J KillianL A Rogowski
Jun 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·W J SwansonC F Aquadro
Feb 12, 2002·Nature Reviews. Genetics·Willie J Swanson, Victor D Vacquier
Nov 9, 2002·Science·Gary T Miller, Scott Pitnick
Jun 14, 2003·Proceedings. Biological Sciences·Lukas Schärer, Peter Ladurner
Apr 1, 1962·Canadian Journal of Microbiology·R R GUILLARD, J H RYTHER
Apr 28, 2004·Journal of Molecular Biology·Lukas KällErik L L Sonnhammer
Apr 30, 2004·Protein Engineering, Design & Selection : PEDS·Jannick Dyrløv BendtsenSøren Brunak
Sep 3, 2004·Evolution; International Journal of Organic Evolution·Catherine L Fry, Gerald S Wilkinson
Nov 20, 2004·Histochemistry and Cell Biology·Peter LadurnerReinhard Rieger
Sep 20, 2005·PLoS Genetics·Nathaniel L Clark, Willie J Swanson
Jan 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Nathaniel L ClarkWillie J Swanson
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E L Charnov
May 20, 2006·Genome Biology·Bartosz Pilch, Matthias Mann
May 30, 2006·Molecular Biology and Evolution·José A AndrésRichard G Harrison
Jun 17, 2006·Proceedings. Biological Sciences·Ronald Chase, Katrina C Blanchard
Oct 19, 2006·Insect Biochemistry and Molecular Biology·Susan J Davies, Tracey Chapman
Oct 31, 2006·Insect Molecular Biology·A M CollinsJ D Evans
Dec 26, 2006·Journal of Insect Physiology·Preethi Radhakrishnan, Phillip W Taylor
Feb 16, 2007·Evolution; International Journal of Organic Evolution·Verena S BrauerNico K Michiels
Mar 24, 2007·Bioinformatics·Triinu Koressaar, Maido Remm
May 1, 2007·Journal of Evolutionary Biology·D B Vizoso, L Schärer
Nov 23, 2007·Molecular Biology and Evolution·Steven A RammRichard D Emes
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
May 23, 2008·Proceedings. Biological Sciences·Jose A AndrésRichard G Harrison
Jun 17, 2008·Animal Reproduction Science·Maria Kareskoski, Terttu Katila
Aug 1, 2008·PLoS Biology·Geoffrey D FindlayWillie J Swanson
Feb 7, 2009·Journal of Evolutionary Biology·T Janicke, L Schärer
Feb 28, 2009·Evolution; International Journal of Organic Evolution·Lukas Schärer
Mar 31, 2009·Insect Biochemistry and Molecular Biology·Erin S KelleherTherese A Markow
Apr 14, 2009·Current Biology : CB·Stuart WigbyTracey Chapman
Apr 9, 2010·Molecular Biology and Evolution·James R Walters, Richard G Harrison

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Citations

Nov 9, 2019·Journal of Evolutionary Biology·Bahar Patlar, Steven A Ramm
Feb 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Julia WundererPeter Ladurner
Oct 20, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Steven A Ramm
Dec 17, 2019·Current Biology : CB·Bahar PatlarSteven A Ramm
Feb 18, 2021·Biological Reviews of the Cambridge Philosophical Society·Peter A DaveyNick Aldred

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Methods Mentioned

BETA
RNA-Seq
PCR

Software Mentioned

Leica LAS
Zoom Browser EX
SecretomeP
BLASTx
ProtComp
ORF Finder
Blast2Go
TMHMM
Blast
SignalP

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