A TCM formula comprising Sophorae Flos and Lonicerae Japonicae Flos alters compositions of immune cells and molecules of the STAT3 pathway in melanoma microenvironment

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
Yu-Xi LiuZhi-Ling Yu

Abstract

A traditional Chinese medicine (TCM) formula (SL) comprising Sophorae Flos and Lonicerae Japonicae Flos was used for treating melanoma in ancient China. We have previously shown that an ethanolic extract of SL (SLE) possesses anti-melanoma effects and suppresses STAT3 signaling in vitro and in vivo. STAT3 has been linked to the development of melanoma immunosuppressive microenvironment. In this work, we investigated whether SLE inhibits melanoma growth by reprogramming the tumor microenvironment in mouse and co-culture cell models. In B16F10 melanoma-bearing mice, we found that intragastric administration of SLE (1.2 g/kg) dramatically inhibited tumor growth. This observation was associated with the downregulation of protein levels of phospho-STAT3 (Tyr 705) and STAT3-regulated immunosuppressive cytokines, and mRNA levels of STAT3-targeted genes involved in tumor growth and immune evasion. We also observed increased Th, Tc and dendritic cells in the melanomas and spleens in SLE-treated mice compared to that in control mice. In a co-culture system composed of B16F10 cells and mouse primary splenic lymphocytes, it was found that SLE not only inhibited STAT3 activation in B16F10 cells, but also downregulated mRNA levels of STAT3-t...Continue Reading

Citations

Apr 22, 2020·Cell Death & Disease·Xiu-Qiong FuZhi-Ling Yu
Jul 14, 2020·Phytotherapy Research : PTR·Chan-Ran ParkNam-Hun Lee
Nov 11, 2019·Chinese Journal of Integrative Medicine·Xin-Bing Sui, Tian Xie
Nov 18, 2020·The American Journal of Chinese Medicine·Sumei WangWanyin Wu
Apr 18, 2021·The Journal of Pharmacy and Pharmacology·Bo CaoLi Han
Aug 28, 2021·Pharmacology & Therapeutics·Luying ZhangVojtech Adam

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