PMID: 7021683Jan 1, 1981Paper

A technique for preparing defined conjugates of horseradish peroxidase and immunoglobulin

Journal of Immunological Methods
P NilssonM S Grundy

Abstract

Conjugates of horseradish peroxidase (HRP) and sheep anti-human immunoglobulin (anti-Ig) were prepared by means of a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). The molar ratios of the conjugates could be changed by varying the degree of chemical substitution of anti-Ig and HRP. Gel filtration and affinity chromatography were used to separate conjugated anti-Ig from free HRP and unconjugated anti-Ig. The distribution of complex sizes and the presence of free HRP and anti-Ig in the final products were monitored by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis. After final purification and characterisation conjugates were studied as reagents in enzyme immunoassays (EIA) with various antigens. Conjugates prepared by SPDP bridging were shown to yield more than 60% of the anti-Ig as conjugated small defined enzyme-protein complexes. A suitably labelled conjugate reacted in a standardised way resulting in a reproducible dose-response curve when a positive serum was assayed in different dilutions.

References

Sep 1, 1975·Journal of Immunological Methods·R Maiolini, R Masseyeff
Jan 1, 1978·Journal of Immunological Methods·E S RectorA H Sehon
Jun 1, 1977·Immunochemistry·J L Guesdon, S Avrameas
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, A Kawaoi
Nov 1, 1968·The Journal of Experimental Medicine·B Bloth, R Bergquist
Sep 1, 1968·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky
Jun 24, 1971·FEBS Letters·B K. Van Weemen, A H.W.M. Schuurs
Dec 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P K Nakane, G B Pierce

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Citations

Oct 12, 1984·Journal of Immunological Methods·R H YolkenM Van Regenmortel
Jul 22, 1988·Journal of Immunological Methods·A JeansonB Rentier
Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·M S GrundyD Warhurst
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·A IslamG Huldt
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·H J AhmedG Huldt
Jun 1, 1994·Enzyme and Microbial Technology·O RyanC O Fágáin
Jan 1, 1983·Journal of Virological Methods·I Ionescu-MatiuG R Dreesman
Jan 1, 1985·Journal of Virological Methods·H G PereiraL De Castro
Aug 1, 1986·Journal of Virological Methods·K Mortensson-Egnund, E Kjeldsberg
Mar 14, 2009·Nature Protocols·Ramadan A AbukneshaZakeya Baalawy
Jan 1, 1991·Preparative Biochemistry·M C SekharP U Sarma
Aug 1, 1982·European Journal of Biochemistry·R ThorstenssonR Norberg
Jan 11, 2002·Molecular and Cellular Biology·David W NeesJoram Piatigorsky
Jan 1, 1992·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·B AlphaC N Hales
Jan 1, 1989·Scandinavian Journal of Infectious Diseases·I Ljungström, F van Knapen
Jan 1, 1989·Scandinavian Journal of Infectious Diseases·K AhlforsE Forsberg
Feb 24, 2007·Cell Biology International·M V MikaelyanV K Gasparyan
Jan 1, 1984·Neuropathology and Applied Neurobiology·I F RoweM B Pepys
Mar 1, 1985·Parasite Immunology·S MeriE Linder
Jan 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P L JansenD F Meijer
Jun 1, 1988·Annals of Tropical Medicine and Parasitology·B EvengårdP Lundbergh
May 1, 1983·Journal of Clinical Microbiology·M S GrundyG Huldt

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