A technique for standardization and quality control of subjective sperm motility assessments in semen analysis

Fertility and Sterility
C H YeungE Nieschlag

Abstract

To establish a quality control method to monitor and eventually to standardize the subjective assessment of sperm motility in conventional semen analysis. Quality control study running over 2 years. University infertility clinic and andrology laboratory. Randomly chosen patients attending the clinic. Conventional semen analysis with sperm motility assessed by grading according to the World Health Organization (WHO) criteria and analysis of individual sperm tracks with a computer-aided sperm analysis (CASA) system. Formulas were established to identify, from the track data, the threshold velocity values for distinguishing between motility grades a and b and motility grades b and c for each technician. The subjective thresholds above which technicians categorized sperm as WHO grades a and b were determined by CASA to be 61 +/- 1 and 11 +/- 1 micron/s (straight-line velocity), respectively. Agreement among three to five technicians over 2 years was reasonable (coefficient of variation < 20%), but threshold values were variable. Agreement within and between laboratories in the assessment of sperm motility grades could be achieved by agreeing on designated values for threshold velocities for grade a and b sperm. On the basis of such...Continue Reading

Citations

Sep 25, 2002·International Journal of Andrology·T G CooperE Nieschlag
Jun 20, 2002·Reproduction in Domestic Animals = Zuchthygiene·Tom RijsselaereA de Kruif
Jan 30, 2010·Asian Journal of Andrology·David J Handelsman, Trevor G Cooper
Nov 19, 2013·Journal of Assisted Reproduction and Genetics·J LammersT Fréour
Apr 11, 2008·International Journal of Andrology·Thomas FreourPaul Barriere
Mar 14, 2007·Reproduction in Domestic Animals = Zuchthygiene·T RijsselaereA Van Soom
Jan 18, 2006·Fertility and Sterility·Trevor G Cooper, Ching-Hei Yeung
Dec 16, 2004·Reproduction in Domestic Animals = Zuchthygiene·P VytA Van Soom
Dec 20, 2011·Systems Biology in Reproductive Medicine·Thomas FréourPaul Barriere
Jun 11, 2005·Theriogenology·T RijsselaereA de Kruif
Mar 14, 2018·Reproduction, Fertility, and Development·Marc YesteMaria M Rivera Del Álamo
Aug 24, 2016·Journal of Korean Medical Science·Ji Ho ChoiChol Shin
Sep 14, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·J AguilarJ A Castilla
Aug 9, 2006·Reproductive Medicine and Biology·Kazuhiko KomoriAkihiko Okuyama
Jan 29, 2004·Reproductive Medicine and Biology·Yuki HiranoMitsuaki Suzuki

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