A Temperature-Gated Nanovalve Self-Assembled from DNA to Control Molecular Transport across Membranes

ACS Nano
Patrick M Arnott, Stefan Howorka

Abstract

Nanopores are powerful nanodevices that puncture semifluid membranes to enable transport of molecular matter across biological or synthetic thin layers. Advanced nanopores featuring more complex functions such as ambient sensing and reversible channel opening are of considerable scientific and technological interest but challenging to achieve with classical building materials. Here we exploit the predictable assembly properties of DNA to form a multifunctional nanovalve that senses temperature for controlled channel opening and tunable transport. The barrel-shaped valve is formed from solely seven oligonucleotides and is closed at ambient temperatures. At >40 °C a programmable thermosensitive lid opens the barrel to allow transport of small molecules across the membrane. The multifunctional DNA nanodevice may be used to create logic ionic networks or to achieve controlled drug delivery from vesicles.

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Citations

Dec 11, 2019·Genes·Es DarleyMatthew A B Baker
Jan 15, 2020·Advanced Biosystems·Qi ShenChenxiang Lin
Aug 23, 2020·Micromachines·Kan Shoji, Ryuji Kawano
Nov 25, 2020·Angewandte Chemie·Conor LanphereStefan Howorka
Jan 19, 2021·Advanced Healthcare Materials·Lingyu FengJianlei Shen
Dec 23, 2020·Nature Protocols·Conor LanphereStefan Howorka
Jul 15, 2021·Advanced Science·Shasha LuXiurong Yang
Aug 10, 2021·International Journal of Biological Macromolecules·Mirza Muhammad Faran Ashraf BaigChengfei Zhang
Dec 10, 2019·ACS Applied Materials & Interfaces·Liang ChenYuanqing Zhang
May 7, 2020·Nano Letters·Diana SobotaUlrich F Keyser
Aug 25, 2021·Chemistry, an Asian Journal·Shi-Yi ZhangRuo-Can Qian
Oct 2, 2021·ACS Nano·Tim DiederichsStefan Howorka
Jan 7, 2022·Chemistry, an Asian Journal·Jing WangDe-Ming Kong
Jan 22, 2022·ACS Applied Bio Materials·Zhongdong WuHao Pei

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