Apr 1, 1973

A temperature-sensitive mutation affecting 28S ribosomal RNA production in mammalian cells

Proceedings of the National Academy of Sciences of the United States of America
D TonioloC Basilico

Abstract

We have characterized a temperature-sensitive (ts) mutant of the hamster cell line BHK 21 that appears to have a defect in the processing of ribosomal RNA precursors at 39 degrees . Mutant ts 422E grows at a normal rate at 33 degrees , but upon shift to 39 degrees growth stops after about one cell doubling. The appearance of 28S rRNA and large ribosomal subunits in the cytoplasm of ts 422E at 39 degrees is inhibited by about 95%, when compared to wild-type BHK cells. Production of 18S rRNA and small ribosomal subunits is unaffected. Shift-up experiments show that the defect in 28S rRNA production can be detected as early as 2-3 hr after the shift to 39 degrees . Synthesis of the larger rRNA precursor is normal at high temperature, but the processing appears to be arrested after the formation of 32S rRNA. 32S rRNA accumulates to some extent in the nucleoli of ts 422E. ts 422E cells appear to have a single mutation, directly affecting the conversion of 32S to 28S rRNA. The reduced amount of 28S rRNA in the cytoplasm of ts 422E cells at 39 degrees seems therefore responsible for their inability to grow at this temperature.

  • References11
  • Citations47

References

Mentioned in this Paper

Centrifugation, Density Gradient
SDS-PAGE
Protoplasm
Kidney
Tritium
Cell Nucleolus
15S RNA
Cricetus
Metazoa
Cell Fractionation

About this Paper

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.