Abstract
Cultures of ts BN75, a temperature-sensitive mutant of BHK 21 cells, show a gradual biphasic drop in [3H]thymidine incorporation together with an accumulation of cells having a G2 DNA content when incubated at 39.5 degrees. However, when higher (41 degrees - 42 degrees) nonpermissive temperatures were used, the major block was in S-phase DNA synthesis. The cultures of ts BN75 shifted to 42 degrees at the start of the S phase, cell-cycle progress was arrested in the middle of S, while under these conditions wild-type BHK cells underwent at least one cycle of DNA synthesis. When ts BN75 cells growth-arrested at high temperature with a G2 DNA content were shifted to the permissive temperature (33.5 degrees C), the restart of DNA synthesis preceded the appearance of mitotic cells. These data suggest that the ts defect of ts BN75 cells might affect primarily the S phase of the cycle rather than the G2 phase.
References
Jul 1, 1975·The Journal of Cell Biology·A Krishan
Nov 1, 1976·Journal of the National Cancer Institute·A P Rao, P N Rao
Jan 1, 1976·Advances in Genetics·D M Prescott
Jan 1, 1977·Advances in Cancer Research·C Basilico
May 1, 1978·Somatic Cell Genetics·T Nishimoto, C Basilico
Oct 1, 1978·Cell·T NishimotoC Basilico
Jan 1, 1977·Journal of Cellular Physiology·R MarzJ R Sheppard
Feb 1, 1974·British Journal of Cancer·D N WheatleyK Kajiwara
Apr 1, 1973·Journal of Cellular Physiology·I E Scheffler, G Buttin
Dec 1, 1974·Journal of Cellular Physiology·S J BurstinC Basilico
Nov 1, 1943·Genetics·S E Luria, M Delbrück
Oct 13, 1971·Nature: New Biology
Citations
Mar 1, 1982·Somatic Cell Genetics·A A McCracken
Sep 1, 1982·Somatic Cell Genetics·D J Chen, R J Wang
Feb 22, 2008·Molecular Biology of the Cell·Yi LuanChuan-Ju Liu
Aug 4, 2004·Genes & Development·Mei-Ling KuoCharles J Sherr
Apr 14, 2010·Molecular and Cellular Biology·Daniel P StewartJoseph T Opferman
Feb 4, 2010·PloS One·Marco MangoneWinship Herr
Dec 1, 1984·Experimental Cell Research·D J Chen, R J Wang
Apr 1, 1987·Experimental Cell Research·T SekiguchiT Nishimoto
May 1, 1984·Cell·A CiechanoverA Varshavsky
Nov 1, 1981·Journal of Cellular Physiology·T NishimotoT Takahashi
Nov 30, 1989·Biochemical and Biophysical Research Communications·Y NishimuneM Sakuda
Apr 30, 1992·Biochemical and Biophysical Research Communications·H NishitaniT Nishimoto
Feb 1, 1988·Archives of Biochemistry and Biophysics·T EkiM Yamada
Oct 9, 2003·Cell·Joanna BloomMichele Pagano
Jun 30, 1988·Biochemical and Biophysical Research Communications·M Ohtsubo, T Nishimoto
Oct 1, 1982·Journal of Cellular Physiology·J Hirschberg, M Marcus
Mar 25, 2006·The Journal of Biological Chemistry·Bo DingPeter Lengyel
Jul 1, 1987·Experimental Cell Research·T EkiM Yamada