PMID: 6160626Jul 1, 1980Paper

A temperature-sensitive mutation affecting S-phase progression can lead to accumulation of cells with a G2 DNA content

Somatic Cell Genetics
T NishimotoC Basilico

Abstract

Cultures of ts BN75, a temperature-sensitive mutant of BHK 21 cells, show a gradual biphasic drop in [3H]thymidine incorporation together with an accumulation of cells having a G2 DNA content when incubated at 39.5 degrees. However, when higher (41 degrees - 42 degrees) nonpermissive temperatures were used, the major block was in S-phase DNA synthesis. The cultures of ts BN75 shifted to 42 degrees at the start of the S phase, cell-cycle progress was arrested in the middle of S, while under these conditions wild-type BHK cells underwent at least one cycle of DNA synthesis. When ts BN75 cells growth-arrested at high temperature with a G2 DNA content were shifted to the permissive temperature (33.5 degrees C), the restart of DNA synthesis preceded the appearance of mitotic cells. These data suggest that the ts defect of ts BN75 cells might affect primarily the S phase of the cycle rather than the G2 phase.

References

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Jan 1, 1977·Advances in Cancer Research·C Basilico
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Oct 13, 1971·Nature: New Biology

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Citations

Aug 4, 2004·Genes & Development·Mei-Ling KuoCharles J Sherr
Apr 14, 2010·Molecular and Cellular Biology·Daniel P StewartJoseph T Opferman
Feb 4, 2010·PloS One·Marco MangoneWinship Herr
Jun 30, 1988·Biochemical and Biophysical Research Communications·M Ohtsubo, T Nishimoto
Oct 1, 1982·Journal of Cellular Physiology·J Hirschberg, M Marcus

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