A ternary quenching electrochemiluminescence insulin immunosensor based on Mn2+ released from MnO2 @Carbon core-shell nanospheres with ascorbic acid quenching AuPdPt-MoS2 @TiO2 enhanced luminol

Biosensors & Bioelectronics
Xiaojian LiQin Wei

Abstract

This work reported a ternary quenching strategy based on electrochemiluminescence resonance energy transfer (ECL-RET) between luminol/H2O2 system and MnO2 capped carbon nanospheres (MnO2@C) and Mn2+ released from the reduction of MnO2 and ascorbic acid (AA) consumed superoxide radical (O2•-) for detection of insulin. To improve the ECL behavior of luminol, AuPdPt hybrid nanoparticles decorated MoS2 nanosheet-coated TiO2 nanobelt (AuPdPt-MoS2@TiO2) were applied to combine with luminol and primary antibodies via covalent linkage which exhibited perfectly catalysis for the electroreduction of H2O2. In order to sensitively detect insulin, labels MnO2@C were used to anchor secondary antibodies via electrostatic interaction and inhibit the ECL intensity of luminol. The perfect overlap of the ECL spectrum of luminol with the absorption spectrum of MnO2 allowed the ECL-RET to decrease ECL signals of luminol. Specifically, AA was introduced not only to inhibit the ECL of luminol, but also to reduce MnO2 producing Mn2+ which can be reacted with O2•- to further quench the ECL reaction between luminol and H2O2. On the basis of the dual quenching effect of luminol, the sandwich immunosensor was prepared to determine insulin with wide linear...Continue Reading

Citations

Mar 4, 2020·Topics in Current Chemistry·Xiangui MaGuobao Xu
Nov 9, 2020·Biomaterials·Si MengS Meng
Feb 4, 2021·Analytical Methods : Advancing Methods and Applications·Mohammad Ali KamyabiMohsen Moharramnezhad
Jun 7, 2021·Analytica Chimica Acta·Qing LiQuan-Guo He

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.