A test for neutrality of mutations of the lacZ transgene

Environmental and Molecular Mutagenesis
L Cosentino, J A Heddle

Abstract

Neutral mutations are stable alterations in DNA that are neither beneficial nor damaging to the cell. Once a mutation is fixed in the DNA, the mutant frequency should increase to its maximum and plateau thereafter, provided that enough expression time is given. Evidence has been obtained indicating neutrality for the endogenous Dlb-1 locus and for the lacl in the BigBlue transgenic system, and is reported here for lacZ in the Muta Mouse system. We have found that, after an acute dose of ethylnitrosourea (ENU, 250 mg/kg) the mutant frequency for both lacZ transgene and Dlb-1 remained constant between 10 and 70 days after mutagenesis in the small intestine, where cell turnover is rapid (approximately 7 days). The same concept should apply to other tissues.

References

May 1, 1994·Mutagenesis·K S Tao, J A Heddle
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K S TaoJ A Heddle
May 1, 1993·Mutagenesis·J C MirsalisJ M Short
Nov 1, 1995·Mutagenesis·J A HeddleX B Zhang
Dec 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L CosentinoJ A Heddle

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Citations

Oct 3, 2001·ILAR Journal·R N Winn
Oct 26, 2000·Environmental Health Perspectives·P M Iannaccone
Oct 18, 2000·Proceedings of the National Academy of Sciences of the United States of America·R N WinnS L Muller
Mar 9, 2010·Drug Metabolism Reviews·Poh-Gek Forkert
Jun 2, 1998·Drug Metabolism Reviews·J A Heddle
Jun 26, 2001·Environmental and Molecular Mutagenesis·R R SwigerJ A Heddle
Jan 18, 2018·International Journal of Molecular Sciences·Ahmad BesaratiniaStella Tommasi
Mar 29, 2000·Environmental and Molecular Mutagenesis·J A HeddleN Yajima
Jan 1, 1996·Environmental and Molecular Mutagenesis·N J Gorelick, J C Mirsalis
Jan 1, 1996·Environmental and Molecular Mutagenesis·J A HeddleY Paashuis-Lew
Jan 6, 2007·International Journal of Cancer. Journal International Du Cancer·Lya G Hernandez, Poh-Gek Forkert
Aug 5, 2011·International Journal of Cancer. Journal International Du Cancer·Joseph B GuttenplanKaram El-Bayoumy
Jul 12, 2019·Emergency Medicine Journal : EMJ·Sarah Edwards, Damian Roland
Oct 7, 2011·Environmental and Molecular Mutagenesis·Christine L LemieuxPaul A White
Jan 29, 2003·Environmental and Molecular Mutagenesis·John A HeddleGeorge R Douglas
May 4, 2011·Environmental and Molecular Mutagenesis·Wieslawa KosinskaJoseph B Guttenplan
Apr 4, 2017·Environmental and Molecular Mutagenesis·Takafumi KimotoBevin P Engelward
Sep 19, 2017·Environmental and Molecular Mutagenesis·Takafumi NakayamaShizuko Kakinuma
Oct 18, 2000·Mutation Research·L Cosentino, J A Heddle
Aug 18, 2000·Mutation Research·W Cruz-MunozJ A Heddle
Oct 11, 2003·Mutation Research·Véronique ThybaudNobuhiro Yajima
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas

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