PMID: 61103Aug 1, 1976Paper

A test of granulocyte membrane integrity and phagocytic function

Cryobiology
F Dankberg, M D Persidsky

Abstract

An assay of granulocyte viability has been developed which yields information about rwo important cell parameters, cell membrane integrity and phagocytic activity. The assay is based on the fact that only live cells can accumulate fluorescein, which is enzymatically split from the nonfluorescent substrate fluorescein diacetate. Dead cells, on the other hand, become permeable to the fluorescent red dye ethidium bromide. When cells are exposed first to opsonized zymosan particles, which they can phagocytize, then to a combination of these fluorescent dyes, one can distinguish microscopically between dead cells with fluorescent red nuclei, live cells which fluoresce green, and live cells with phagocytic function which are swollen with the pink zymosan particles in a green fluorescing cytoplasm. This assay takes 20--30 min and can be used to distinguish different degrees of cellular damage after cryopreservation.

References

Jan 1, 1974·Vox Sanguinis·J L MartelP Rubinstein
Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·B Rotman, B W Papermaster

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Citations

Jun 1, 1986·Cell Biology and Toxicology·M AeschbacherG Zbinden
Apr 16, 1982·Molecular and Cellular Biochemistry·B D DaviesF Lionetti
Jan 1, 1984·Journal of Inherited Metabolic Disease·M Di RoccoF Patrone
Jan 1, 1981·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·F PatroneG Lanzi
Jan 1, 1980·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·F PatroneC Sacchetti
Jan 1, 1980·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·F PatroneC Sacchetti
Jun 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M P Carson, C C Haudenschild
Jun 1, 1977·Cryobiology·M D Persidsky, G S Baillie
Feb 28, 2004·Il Farmaco·Olga BrunoFranco Dallegri
Nov 1, 1978·British Journal of Industrial Medicine·D Mangan, I S Snyder
Apr 26, 2001·Analytical Biochemistry·S Sesták, V Farkas
Jun 22, 2012·Biotechnology Progress·Rita MalpiquePaula M Alves
Aug 1, 1978·Cryobiology·F L DankbergL S Olson
May 1, 1992·The Journal of Surgical Research·J A FreischlagW S Moore
Nov 15, 1994·Thrombosis Research·L T OsnesP Kierulf
Feb 1, 1986·The Journal of Surgical Research·J FreischlagR W Busuttil
Jun 1, 1991·The Journal of Surgical Research·J A Freischlag, D Hanna
Sep 1, 1987·The Journal of Surgical Research·F C ShengR W Busuttil
Feb 1, 1983·Scandinavian Journal of Immunology·F DallegriC Sacchetti
Jan 1, 1984·British Journal of Haematology·F DallegriC Sacchetti
Jul 15, 1986·Biochemical Pharmacology·W HossB D Davies
Nov 27, 1991·Biochemical Pharmacology·L OttonelloC Sacchetti

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