PMID: 8989328Jan 1, 1997Paper

A test of the role of the proximal histidines in the Perutz model for cooperativity in haemoglobin

Nature Structural Biology
D BarrickC Ho

Abstract

Human haemoglobin has long been a paradigm for cooperative ligand binding and allostery. Through analysis of the crystal structures of deoxyhaemoglobin and liganded haemoglobin, Perutz proposed a model for cooperativity in which the bond between the proximal histidine and the protein couples haem rearrangements to protein structure rearrangements. Here we test this model by deleting the bonds between the histidine imidazole side chain and the polypeptide. This detachment method allows us to determine directly the contribution of proximal histidine coupling to cooperativity of distal ligand binding. Proximal detachment significantly increases ligand affinity, reduces cooperativity, and prevents quaternary structure switching, in accord with the Perutz model. Residual cooperativity indicates that additional haem communication pathways exist that do not involve the proximal histidine coupling mechanism.

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Citations

Sep 11, 2002·Journal of Molecular Biology·Yue YuanChien Ho
Jul 20, 2002·Biophysical Chemistry·Ching-Hsuan Tsai, Chien Ho
May 10, 2001·Trends in Biochemical Sciences·W E RoyerH A Heaslet
Aug 21, 2003·Trends in Plant Science·Suman KunduMark S Hargrove
Mar 4, 2005·The European Physical Journal. E, Soft Matter·D PozziA Congiu Castellano
Jul 1, 1998·Annual Review of Biophysics and Biomolecular Structure·M F PerutzG G Dodson
May 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shin-Ichi AdachiNaoya Shibayama
Jan 6, 2017·Cell Death & Disease·Marta CodrichStefano Gustincich
Mar 14, 2007·Biophysical Journal·Bogdan M LeuJ Timothy Sage
Apr 15, 2009·Biopolymers·Carmelinda SavinoBeatrice Vallone
Jun 29, 2001·The Journal of Biological Chemistry·J T TrentM S Hargrove
May 12, 1998·Hemoglobin·T H Huisman, M F Carver
Mar 11, 2004·Chemical Reviews·Jonathan A Lukin, Chien Ho
Jan 22, 2015·Chemical Reviews·Yue YuanChien Ho

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