A tetracycline inducible expression vector for Corynebacterium glutamicum allowing tightly regulable gene expression

Plasmid
Frank LausbergRoland Freudl

Abstract

Here we report on the construction of a tetracycline inducible expression vector that allows a tightly regulable gene expression in Corynebacterium glutamicum which is used in industry for production of small molecules such as amino acids. Using the green fluorescent protein (GFP) as a reporter protein we show that this vector, named pCLTON1, is characterized by tight repression under non-induced conditions as compared to a conventional IPTG inducible expression vector, and that it allows gradual GFP synthesis upon gradual increase of anhydrotetracycline addition.

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Citations

Jan 12, 2013·Microbial Biotechnology·Miroslav PátekJan Nešvera
Oct 16, 2016·Critical Reviews in Biotechnology·Xiuxia LiuZhonghu Bai
Sep 3, 2013·Applied and Environmental Microbiology·Kristina HoffmannLothar Eggeling
Jan 22, 2019·Applied Microbiology and Biotechnology·Xiaoyuan Wang
Jun 3, 2021·ACS Synthetic Biology·Alina BurmeisterAlexander Grünberger
Oct 12, 2021·Frontiers in Bioengineering and Biotechnology·Rahul GauttamBernhard J Eikmanns
Oct 30, 2021·Microbial Biotechnology·Ralph BertramChristopher F Schuster

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