A theoretical model of the tridimensional structure of Bacillus thuringiensis subsp. medellin Cry 11Bb toxin deduced by homology modelling

Memórias do Instituto Oswaldo Cruz
P GutierrezSergio Orduz

Abstract

Cry11Bb is an insecticidal crystal protein produced by Bacillus thuringiensis subsp. medellin during its stationary phase; this partial differential-endotoxin is active against dipteran insects and has great potential for mosquito borne disease control. Here, we report the first theoretical model of the tridimensional structure of a Cry11 toxin. The tridimensional structure of the Cry11Bb toxin was obtained by homology modelling on the structures of the Cry1Aa and Cry3Aa toxins. In this work we give a brief description of our model and hypothesize the residues of the Cry11Bb toxin that could be important in receptor recognition and pore formation. This model will serve as a starting point for the design of mutagenesis experiments aimed to the improvement of toxicity, and to provide a new tool for the elucidation of the mechanism of action of these mosquitocidal proteins.

References

Jan 1, 1992·Annual Review of Entomology·S S GillP V Pietrantonio
Jan 1, 1992·Journal of Invertebrate Pathology·S OrduzH de Barjac
Mar 1, 1990·Journal of Molecular Graphics·G Vriend
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Z GeD H Dean
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C HofmannH Van Mellaert
Dec 1, 1995·Journal of Molecular Biology·P GrochulskiM Cygler
Oct 29, 1993·Biochemical and Biophysical Research Communications·F S WaltersL H English
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·X J ChenD H Dean
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·N CrickmoreD H Dean
May 29, 1999·Applied and Environmental Microbiology·A I AronsonL Wu
Aug 30, 2000·Journal of Invertebrate Pathology·C SeguraS Orduz
Jul 1, 1995·Applied and Environmental Microbiology·F FeldmannC Waalwijk

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Citations

May 28, 2008·Journal of Molecular Modeling·Li-Qiu XiaYun-Jun Sun
Jan 17, 2009·Biochimica Et Biophysica Acta·Mauricio AriasVictor V Lemeshko
Feb 17, 2005·The Journal of Biological Chemistry·Victor V LemeshkoSergio Orduz
Oct 23, 2012·Bioscience Reports·Victor V Lemeshko, Sergio Orduz
May 15, 2002·Bioscience, Biotechnology, and Biochemistry·Masashi YamagiwaHiroshi Sakai
Sep 30, 2008·Molecular Biology Reports·Dil Ara Abbas Bukhari, Abdul Rauf Shakoori
Sep 1, 2016·BMC Bioinformatics·David BuzattoSônia Marli Zingaretti
Aug 13, 2020·Evolutionary Bioinformatics Online·Efraín Hernando Pinzón-ReyesPaola Rondón-Villareal
Apr 27, 2021·Archives of Biochemistry and Biophysics·Santiago Rendon-MarinSergio Orduz
Jul 3, 2021·Toxins·Guillaume TetreauJacques-Philippe Colletier

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